Multiple Gene Expression in Cell-Free Protein Synthesis Systems for Reconstructing Bacteriophages and Metabolic Pathways.

As a fast and reliable technology with applications in diverse biological studies, cell-free protein synthesis has become popular in recent decades. The cell-free protein synthesis system can be considered a complex chemical reaction system that is also open to exogenous manipulation, including that which could otherwise potentially harm the cell's viability. On the other hand, since the technology depends on the cell lysates by which genetic information is transformed into active proteins, the whole system resembles the cell to some extent.

A statistical approach for optimization of alkaline lipase production by ascidian associated- RSK CAS9.

A marine ascidian-associated bacterium, RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.

Molecular identification of ascidians from the Palk Bay Region, Southeast coast of India.

Background: More than 50 ascidian species distributed in the Palk Bay, Southeast coat of India. Up to a very few molecular work has been performed to determine the evolutionary relationships of ascidians in the world.

Objectives: Present study explored the value of mtDNA data in assessing phylogenetic relationships within the family Ascidiacea, Didemnidae, Styelidae, and molecular identification of ascidians from the Palk Bay, Southeast coast of India.