Uncloaking cell-impermeant gold nanorods via tumor microenvironmental cathepsin B facilitates cancer cell penetration and potent radiosensitization.

Major impediments to conveyance of intravenously administered drugs to tumors are biofouling, opsonization, and rapid clearance from the circulation by macrophages and reticuloendothelial phagocytes. Cloaking nanoparticles with stealth epilayers partly overcomes these hurdles but it also foils interactions with tumor cells. Here, we describe the synthesis, characterization, and validation of smart gold nanorods (GNRs) that spontaneously transform from inert passengers in the blood stream to active cell-penetrating nanoparticles within tumors to potently sensitize tumors to radiation therapy.

Molecular organization of the early stages of nucleosome phase separation visualized by cryo-electron tomography.

It has been proposed that the intrinsic property of nucleosome arrays to undergo liquid-liquid phase separation (LLPS) in vitro is responsible for chromatin domain organization in vivo. However, understanding nucleosomal LLPS has been hindered by the challenge to characterize the structure of the resulting heterogeneous condensates. We used cryo-electron tomography and deep-learning-based 3D reconstruction/segmentation to determine the molecular organization of condensates at various stages of LLPS.

Gold Nanorod Synthesis with Small Thiolated Molecules.

Size and shape tunability have been widely demonstrated for gold nanorods (AuNRs), but reproducible and reliable protocols for the synthesis of small nanocrystals with high yield are still needed for potential biomedical applications. Here, we present novel seed-mediated and seedless protocols for gold nanorods by incorporating bioadditives or small thiolated molecules during the growth stage. The bioadditives glutathione (GSH), oxidized glutathione (GSSG), l-cysteine (l-cys), and l-methionine (l-met) are utilized in nanomolar and micromolar concentrations to modify the aspect ratio of AuNRs in a reproducible form.

High yield synthesis and surface chemistry exchange of small gold hexagonal nanoprisms.

A new seed-mediated synthesis of AuHNPs in high yield is described using hydroquinone as a weak reductant and poly(vinylpyrrolidone) as a shape-directing additive. We obtain distinct and small edge lengths of AuHNPs with long-term shape stability. Also, PVP enhances the monodispersity and enables the higher stability of functionalized nanoprisms.

Improving the Shape Yield and Long-Term Stability of Gold Nanoprisms with Poly(vinylpyrrolidone).

Gold nanoprisms (AuNPRs) are anisotropic nanostructures that have gained great attention in recent years because of their interesting and unique optical properties that can be tailored for biomedical, energy, and sensing applications. At present, several protocols have reported the high yield synthesis of AuNPRs of different dimensions using a seed-mediated approach. However, there is a need to develop reproducible and scalable methods with the goal of a controllable synthesis.

Accelerating Gold Nanorod Synthesis with Nanomolar Concentrations of Poly(vinylpyrrolidone).

A novel modification for the seedless synthesis of gold nanorods (AuNRs) has been developed. Nanomolar concentrations of 10 kDa poly(vinylpyrrolidone) (PVP) can be introduced to a growth solution containing 25, 50, or 100 mM cetyltrimethylammonium bromide (CTAB) to significantly reduce the dimensions of AuNRs. We found that PVP accelerates the growth rate of AuNRs by more than two times that of nanorods grown in 50 and 100 mM CTAB solutions.