Situational diagnosis of plans for the surveillance, prevention and control of COVID-19 at work in the hydrocarbon sector.

Introduction: During the COVID-19 pandemic in Peru, economic activities were restricted to limit the risk of contagion, and companies were required to prepare and register the plan for COVID-19 surveillance, prevention and control in the workplace prior to resuming activities.

Objective: To describe the status of plan registration in the hydrocarbon sector during the first half of 2020, as well as the characteristics of health professionals and occupational health and safety supervisors or committees.

Methods: Cross-sectional study with secondary analysis of databases obtained from the Integrated System for COVID-19 of Companies (Sistema Integrado para COVID-19 de Empresas, SISCOVID Empresas) and government public data on the mining sector.

Extended-spectrum beta-lactamase-producing enterobacterales carrying the mcr-1 gene in Lima, Peru.

We analyzed the presence of the mcr-1 gene in 165 extended-spectrum beta-lactamase-producing enterobacterales (ESBL-PE) obtained during 2017, from blood (40), urine (57), lower respiratory secretions (12) and rectal swabs (56) of patients hospitalized in the Instituto Nacional de Enfermedades Neoplásicas (Peru). Antimicrobial identification and susceptibility were determined by the Phoenix M50 automated system; colistin resistance by Colistin Agar-Spot (CAS); mrc-1 detection by colistin pre-diffusion and inhibition with EDTA test (CPD-E) and by polymerase chain reaction (PCR). We found that from the 165 ESBL-PE, 25 were positive for mcr-1 by the CPD-E method and confirmed by PCR.

Phenotypic Detection of Plasmid-Mediated Colistin Resistance in .

The aim of this work was to evaluate an easy-to-perform assay based upon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92 nonrelated isolates of (74 , 17 , and 1 ). Our proposed method is based on a modification of the colistin agar-spot screening test (CAST), a plate containing 3 μg/ml colistin, by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).

Detection of plasmid-mediated colistin resistance by colistin pre-diffusion and inhibition with EDTA test (CPD-E) in Enterobactereaceae.

A phenotypic assay based on colistin pre-diffusion and differential inhibition of Mobile Colistin Resistance (MCR) protein activity by EDTA showed that, of the 92 strains tested, all MCR producers (49) exhibited an increase ≥5 mm in the inhibition zone around the area of pre-diffusion in the presence of EDTA, in comparison with colistin alone. Results suggest that CPD-E may differentiate MCR-producing microorganisms from resistant microorganisms without this marker.