Publications by authors named "Katherine Lauro"
Article Synopsis
- RecombiCraft is a new and cost-effective method for rapidly creating DNA libraries in E. coli by using SLiCE and liquid culture amplification.
- The approach utilizes E. coli's natural abilities to recombine DNA, streamlining the process by eliminating the need for multiple enzymes and minimizing sequence biases.
- Overall, this technique can generate DNA libraries in approximately 12 hours, making it a valuable tool for genetic research and biotechnology.
Advances of engineered extracellular vesicles-based therapeutics strategy.
Sci Technol Adv Mater
October 2022
Extracellular vesicles (EVs) are a heterogeneous population of lipid bilayer membrane-bound vesicles which encapsulate bioactive molecules, such as nucleic acids, proteins, and lipids. They mediate intercellular communication through transporting internally packaged molecules, making them attractive therapeutics carriers. Over the last decades, a significant amount of research has implied the potential of EVs servings as drug delivery vehicles for nuclear acids, proteins, and small molecular drugs.
View Article and Find Full Text PDFBackground: Extracellular vesicles (EVs) are attracting interest as a new class of drug delivery vehicles due to their intrinsic nature of biomolecular transport in the body. We previously demonstrated that EV surface modification with tissue-specific molecules accomplished targeted EV-mediated DNA delivery.
Methods: Here, we describe reliable methods for (i) generating EGFR tumor-targeting EVs via the display of high-affinity monobodies and (ii) in vitro measurement of EV binding using fluorescence and bioluminescence labeling.