Publications by authors named "Katherine L Lydon"
Article Synopsis
- - The study investigates "meniscons," cells from the meniscus surrounded by their natural matrix, to determine if they can aid in cartilage repair similarly to chondrons, which are known for their regenerative properties.
- - Researchers isolated and cultured human meniscons from osteoarthritic meniscus tissue and compared their behavior and characteristics to chondrons over a 28-day period, finding similarities in cell growth and production of key cartilage components.
- - The findings highlight the potential of meniscons for future therapies in treating meniscus injuries, as they demonstrate comparable biochemical activity to chondrons while being quickly isolated, which could streamline treatment approaches.
Objective: This proof-of-concept study investigated an improved cell-based injection therapy combining mesenchymal stem cells (MSCs) and meniscus cells (MCs) to support superior meniscus allograft repopulation and early revival compared to injecting MSCs alone.
Design: In this controlled laboratory study, frozen meniscus allograft samples were injected vertically with a cell suspension containing different ratios of MSCs and MCs or control (lactated ringers) and cultured for 28 days. Samples were analyzed weekly for cell viability, migration, and metabolism using histological and biochemical assays.
Background: Fresh-frozen allografts are the current standard in meniscal allograft transplant (MAT) surgery, due to their availability, ease of preservation, and affordability. However, fresh-frozen grafts are associated with several clinical challenges such as graft shrinkage and extrusion, among many others.
Purpose: To present the current knowledge on the use of fresh meniscal allografts, presenting whether benefits associated with fresh grafts provide sufficient evidence to support their use in clinical practice.
Matrix stiffness is a central regulator of fibroblast function. However, the transcriptional mechanisms linking matrix stiffness to changes in fibroblast phenotype are incompletely understood. Here, we evaluated the effect of matrix stiffness on genome-wide chromatin accessibility in freshly isolated lung fibroblasts using ATAC-seq.
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