Publications by authors named "Katherine D Long"
Chimerism-Mediated Tolerance in Intestinal Transplantation.
Gastroenterol Clin North Am
September 2024
In this review, the authors outlined concepts and strategies to achieve immune tolerance through inducing hematopoietic chimerism after solid organ transplantation and introduced challenges and opportunities in harnessing two-way alloresponses to improve outcomes after intestinal transplantation (ITx). Next, the authors discussed the dynamics and phenotypes of peripheral blood and intestinal graft T-cell subset chimerism and their association with outcomes. The authors also summarized studies on other types of immune cells after ITx and their potential participation in chimerism-mediated tolerance.
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- The study investigates how B cell populations and their receptors evolve in the intestines of humans, particularly after intestinal transplantation, using biopsies for observation.
- Researchers employed advanced techniques like polychromatic flow cytometry and B cell receptor sequencing to differentiate between donor and recipient B cells and assess their development in the transplanted intestines.
- Findings reveal that recipient B cells, including memory B cells, rapidly populate the transplanted intestines mainly in infants, and their B cell receptors evolve differently in the graft compared to circulation, with notable clonal mixing remaining years after the transplant.
Article Synopsis
- * Researchers analyzed blood, lymphoid, and gut samples from 16 ITx patients using flow cytometry and next-generation sequencing to explore the origins and specificities of TRMs.
- * Findings showed that recipient TRM repertoires in transplanted ileum are influenced by donor age and T cell macrochimerism, with a notable overlap of T cell receptor sequences between gut and blood, suggesting ongoing interactions for years post-transplant.
Article Synopsis
- The study examines how B cell populations and their receptors (BCRs) are formed and maintained in the intestine after intestinal transplantation (ITx).
- Researchers used advanced techniques to analyze B cells from both donors and recipients, revealing that recipient B cells, including memory B cells, quickly established themselves in the transplant's mucosa, especially in infants.
- Despite ongoing changes in B cell populations post-transplant, there is no evidence of a stable B cell repertoire being formed in the gut tissue, even years after the procedure.
Understanding immune cell dynamics after intestinal transplantation has provided new insights into human lymphocyte biology. However, isolating and characterizing such cells can be challenging. Here, we provide a protocol to isolate intraepithelial and lamina propria lymphocytes from human ileal biopsies.
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