Publications by authors named "Katharina Schmied"
Murine organotypic brain slice cultures have been widely used in neuroscientific research and are offering the opportunity to study neuronal function under normal and disease conditions. Despite the broad application, the mechanisms governing the maturation of immature cortical circuits in vitro are not well understood. In this study, we present a detailed investigation into the development of the neocortex in vitro.
View Article and Find Full Text PDFArticle Synopsis
- Investigating the human brain at the cellular level is difficult due to the fragility of neuronal tissue and limited human brain material.
- Brain slices are useful for studying brain functions and diseases over extended periods, as this method overcomes the constraints of acute slices.
- The paper provides a detailed protocol for long-term culturing of human brain slice cultures, discusses challenges in the process, and demonstrates their viability through various recording techniques and viral expression results.