Publications by authors named "Kateryna Karpoff"
Many esophageal diseases can arise during development or throughout life. Therefore, well-characterized in vitro models and detailed methods are essential for studying human esophageal development, homeostasis and disease. Here, we (1) create an atlas of the cell types observed in the normal adult human esophagus; (2) establish an ancestrally diverse biobank of in vitro esophagus tissue to interrogate homeostasis and injury; and (3) benchmark in vitro models using the adult human esophagus atlas.
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- Human colonoids in low-calcium conditions (0.25 mM) can differentiate and express tight junction proteins, but don't express desmosomal proteins; adding calcium increases desmosomal protein expression and desmosome formation.
- In transwell cultures, researchers assessed the impact of calcium on barrier integrity by measuring trans-epithelial electrical resistance (TEER), finding higher TEER values in low-calcium settings that improved with calcium.
- The study concludes that while low calcium supports a permeability barrier, higher calcium levels enhance tissue cohesion and overall barrier function, highlighting the role of calcium intake in colonic health.