Publications by authors named "Katerina Volodina"
Article Synopsis
- Interaction between nanoparticles and biomolecules can lead to either compatible or harmful complexes, but the structural details of these interactions are not well understood.
- This study investigates how a blood protein, bovine serum albumin, behaves when it is adsorbed onto silicon and germanium nanoparticles, especially when heated to 640 K.
- The results show that the protein is more heat-resistant when attached to nanoparticles than in solution or solid, with germanium providing better heat resistance than silicon, which could promote safer use of these nanoparticles in biological applications.
We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method, demonstrated by the renaturation of several enzymes, is efficient, rapid, employs a minimal amount of reagents and even can be applied to renature mixture of the denatured enzymes.
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