The Basis for Strain-Dependent Rat Aldehyde Dehydrogenase 1A7 () Gene Expression.

Aldehyde hydrogenases (ALDHs) belong to a large gene family involved in oxidation of both endogenous and exogenous compounds in mammalian tissues. Among ALDHs, the rat gene displays a curious strain dependence in phenobarbital (PB)-induced hepatic expression: the responsive strains exhibit induction of both ALDH1A7 and CYP2B mRNAs and activities, whereas the nonresponsive strains show induction of CYP2B only. Here, we investigated the responsiveness of , , and genes to prototypical P450 inducers, expression of nuclear receptors CAR and pregnane X receptor, and structure of the promoter in both rat strains.

Monitoring of multiple bacteriocins through a developed dual extraction protocol and comparison of HPLC-DAD with turbidometry as their quantification system.

The present study describes the development of a simple and efficient screening system that allows identification and quantification of nine bacteriocins produced by Lactococcus lactis. Cell-free L. lactis extracts presented a broad spectrum of antibacterial activity, including Gram-negative bacteria, Gram-positive bacteria, and fungi.