Publications by authors named "Katelyn Li"
Human neural organoids, generated from pluripotent stem cells in vitro, are useful tools to study human brain development, evolution and disease. However, it is unclear which parts of the human brain are covered by existing protocols, and it has been difficult to quantitatively assess organoid variation and fidelity. Here we integrate 36 single-cell transcriptomic datasets spanning 26 protocols into one integrated human neural organoid cell atlas totalling more than 1.
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- * By employing single-nucleus RNA sequencing, researchers analyzed over 170,000 astrocyte nuclei across different developmental stages and brain areas in mice and marmosets, revealing distinct regional differences in astrocyte gene expression that are not shared with neurons or other glial cells.
- * The study found that while astrocytes show regional patterns even in late embryonic stages, their gene expression changes significantly after birth for specialization, with differences in gene expression and maturation timing observed between the two species studied. *
Article Synopsis
- High levels of proinflammatory cytokines lead to neurotoxicity and neurodegeneration, but the specific mechanisms of their release from microglia are unclear.
- The study reveals that secretory autophagy (SA) plays a key role in neuroinflammation and neurodegeneration through SKA2 and FKBP5 signaling, with SKA2 preventing excessive release of IL-1β.
- Knocking down Ska2 in male mice results in increased SA activation, causing neuroinflammation and rapid hippocampal atrophy, and findings suggest that hyperactive SA is also present in Alzheimer's disease, indicating a connection between SA and neuroinflammatory processes.
Article Synopsis
- Researchers examined over 2.4 million brain cells from 18 locations in the common marmoset using single-nucleus RNA sequencing to analyze gene expression patterns in various brain structures.
- The findings suggest that the adult identity of most neuron types is influenced more by their developmental origins than by the types of neurotransmitters they release.
- High proportions of specific neuron types were found in higher-order cortical areas, and the study utilized cell type-specific enhancers and AAV-GFP to visualize different interneuron morphologies in the neocortex and striatum.
High levels of proinflammatory cytokines induce neurotoxicity and catalyze inflammation-driven neurodegeneration, but the specific release mechanisms from microglia remain elusive. We demonstrate that secretory autophagy (SA), a non-lytic modality of autophagy for secretion of vesicular cargo, regulates neuroinflammation-mediated neurodegeneration via SKA2 and FKBP5 signaling. SKA2 inhibits SA-dependent IL-1β release by counteracting FKBP5 function.
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