Advancing the application of pXRF for animal samples.

Portable x-ray fluorescent (pXRF) technology provides significant opportunities for rapid, non-destructive data collection in a range of fields of study. However, there are sources of variation and sample assumptions that may influence the data obtained, particularly in animal samples. We used representative species for four taxa (fish, mammals, birds, reptiles) to test the precision of replicate scans, and the impact of sample thickness, sample state, scan location and scan time on data obtained from a pXRF.

Determining the Provenance of Traded Wildlife in the Philippines.

The illegal wildlife trade is a significant threat to global biodiversity, often targeting already threatened species. In combating the trade, it is critical to know the provenance of the traded animal or part to facilitate targeted conservation actions, such as education and enforcement. Here, we present and compare two methods, portable X-ray fluorescence (pXRF) and stable isotope analysis (SIA), to determine both the geographic and source provenance (captive or wild) of traded animals and their parts.

Heavy metal habitat: A novel framework for mapping heavy metal contamination over large-scale catchment with a species distribution model.

Heavy metal(loid)s (HMs) have been consistently entering the food chain, imposing great harm on environment and public health. However, previous studies on the spatial dynamics and transport mechanism of HMs have been profoundly limited by the field sampling issues, such as the uneven observations of individual carriers and their spatial mismatch, especially over large-scale catchments with complex environment. In this study, a novel methodological framework for mapping HMs at catchment scale was proposed and applied, combining a species distribution model (SDM) with physical environment and human variables.

Aerial surveys of waterbirds in Australia.

Tracking long-term environmental change is important, particularly for freshwater ecosystems, often with high rates of decline. Waterbirds are key indicators of freshwater ecosystem change, with groups reflecting food availability (e.g.

Whole-genome sequence analysis of environmental from the faeces of straw-necked ibis () nesting on inland wetlands.

Wildlife, and birds in particular, play an increasingly recognized role in the evolution and transmission of that pose a threat to humans. To characterize these lineages and their potential threat from an evolutionary perspective, we isolated and performed whole-genome sequencing on 11 sequence types (STs) of recovered from the desiccated faeces of straw-necked ibis () nesting on inland wetlands located in geographically different regions of New South Wales, Australia. Carriage of virulence-associated genes was limited, and no antimicrobial resistance genes were detected, but novel variants of an insertion element that plays an important role in capturing and mobilizing antibiotic resistance genes, IS, were identified and characterized.

Duplication and diversification of a unique chromosomal virulence island hosting the subtilase cytotoxin in ST58.

The AB cytotoxins are important virulence factors in . The most notable members of the AB toxin families include Shiga toxin families 1 (Stx) and 2 (Stx), which are associated with enterohaemorrhagic infections causing haemolytic uraemic syndrome and haemorrhagic colitis. The subAB toxins are the newest and least well understood members of the AB toxin gene family.

When all life counts in conservation.

Conservation science involves the collection and analysis of data. These scientific practices emerge from values that shape who and what is counted. Currently, conservation data are filtered through a value system that considers native life the only appropriate subject of conservation concern.

Tracing geochemical pollutants in stream water and soil from mining activity in an alpine catchment.

This research developed a method of tracing major water chemical parameters (WCP) and soil heavy metals (HM) to identify the processes of mining pollution in topographically complex landscapes. Ninety-nine spatially distributed water samples were collected to characterise the hydrochemical characteristics of an alpine river in north-west China. Sixty river WCP and fifty-six soil HM samples from areas near mining sites were then used to analyse the mining pollution process.

Novel detection of provenance in the illegal wildlife trade using elemental data.

Despite being the fourth largest criminal market in the world, no forensic tools have been sufficiently developed to accurately determine the legal status of seized animals and their parts. Although legal trading is permissible for farmed or captive-bred animals, many animals are illegally removed from the wild and laundered by masquerading them as captive bred. Here we present high-resolution x-ray fluorescence (XRF) as a non-invasive and cost-effective tool for forensic classification.