Publications by authors named "Katarzyna Zawieracz"
Article Synopsis
- The study finds that the YTHDF1 protein, which reads N6-methyladenosine on RNA, plays a crucial role in cancer progression and is upregulated in dendritic cells (DCs) after radiotherapy (RT).
- High levels of YTHDF1 in DCs are linked to worse patient outcomes during RT, while removing or inhibiting YTHDF1 boosts the effectiveness of radiation therapy in cancer models.
- The research uncovers a regulatory mechanism between YTHDF1 and the STING signaling pathway, suggesting that targeting YTHDF1 could improve cancer treatment strategies such as RT and radioimmunotherapy.
Cancer-associated fibroblasts (CAFs) play important roles in regulating tumor progression, metastasis, and response to therapies. Accurately modeling the interplay between cancer cells and the tumor microenvironment (TME) requires the use of primary cells from patient samples. Here we describe methods for the isolation of both primary CAFs and fibroblasts from omental tissue using a combination of mechanical dissociation and enzymatic digestion.
View Article and Find Full Text PDFThe growing use of neoadjuvant chemotherapy to treat advanced stage high-grade serous ovarian cancer (HGSOC) creates an opportunity to better understand chemotherapy-induced mutational and gene expression changes. Here we performed a cohort study including 34 patients with advanced stage IIIC or IV HGSOC to assess changes in the tumor genome and transcriptome in women receiving neoadjuvant chemotherapy. RNA sequencing and panel DNA sequencing of 596 cancer-related genes was performed on paired formalin-fixed paraffin-embedded specimens collected before and after chemotherapy, and differentially expressed genes (DEG) and copy-number variations (CNV) in pre- and post-chemotherapy samples were identified.
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