Associations between transient and chronic loneliness, and depression, in the understanding society study.

Objectives: Loneliness has a long-established link with depression; however, patterns of loneliness, specifically transient (short-term) and chronic loneliness (longer-term), have seldom been researched in terms of their associations with depression and psychiatric distress. We investigated whether chronic loneliness could predict higher levels of psychiatric distress and higher chance of depression diagnosis (via self-report) than transient and no loneliness.

Methods: We used data from 18,999 participants in Waves 9 and 10 of the Understanding Society survey: a nationally representative study of adults in the United Kingdom.

Virulence factors, biofilm-forming ability, and antimicrobial resistance of urinary strains isolated from hospitalized patients.

Background/aim: Escherichia coli is the most frequent cause of urinary tract infections. We investigated the possible associations between the origin of strains, antimicrobial resistance, the presence of urovirulence factors, and biofilm-forming ability.

Materials And Methods: Antibiotic susceptibility of E.

Virulence gene profiles in Staphylococcus aureus isolated from cows with subclinical mastitis in eastern Poland.

Staphylococcus aureus is arguably the most important pathogen involved in bovine mastitis. The aim of this study was to determine the virulence gene profiles of 124 Staph. aureus isolates from subclinical mastitis in cows in eastern Poland.

Antibiofilm activity of trans-cinnamaldehyde, p-coumaric, and ferulic acids on uropathogenic Escherichia coli.

Background/aim: Biofilm on urinary catheters results in persistent infections that are resistant to antibiotics. In this study, phytochemicals were assessed as alternative antimicrobials in preventing and inactivating E. coli biofilm on urinary catheters.

Bullous pyoderma gangrenosum associated with pancytopenia of unknown origin.

Pyoderma gangrenosum (PG) is a neutrophilic dermatosis of unknown origin. Clinically it starts with a pustule, nodule or bulla that rapidly progresses and turns into a painful ulcer with raised, undermined borders. The etiopathogenesis of PG remains unknown.

[Resistance of Pseudomonas aeruginosa to antibiotics].

The main problem in the treatment of nosocomial infections is the increasing drug resistance of microorganisms that cause them, limiting the number of effective antibiotics. Pseudomonas aeruginosa bacilli are the cause of many serious hospital-acquired infections occurring primarily in patients within high-risk groups. The most vulnerable are those with weakened immune systems, as well as those with extensive surgical wounds and burn wounds.

Twitching motility activity, biofilm formation, and genetic typing for clinical isolates of Pseudomonas aeruginosa by random amplified DNA PCR.

A total of 44 Pseudomonas aeruginosa clinical strains were studied by random amplified polymorphic DNA PCR. (RAPD)-PCR analysis determined the presence of 15 genotypes, with the most frequent genotype A detected in 27.3% of the strains.

BOX-PCR is an adequate tool for typing of clinical Pseudomonas aeruginosa isolates.

In this study, the BOX-PCR fingerprinting technique was evaluated for the discrimination of clinical Pseudomonas aeruginosa isolates. All isolates were typeable and nearly half showed unique banding patterns. According to our results, BOX-PCR fingerprinting is applicable for typing of Pseudomonas aeruginosa isolates and can be considered a useful complementary tool for epidemiological studies of members of this genus.

Phenotypic and genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in siedlce (Poland).

A total of 62 Pseudomonas aeruginosa strains isolated from two hospitals in Siedlce (Poland) were studied by repetitive element based PCR (rep-PCR) using BOX primer. BOX-PCR results revealed the presence of 7 numerous genotypes and 31 unique patterns among isolates. Generally, the strains of P.

Genetic features of clinical Pseudomonas aeruginosa strains.

The genetic features of each isolate were determined by enterobacterial repetitive intergenic consensus (ERIC) primer sequences used in PCR and by searching for six virulence genes (alg D, las B, tox A, plc H, plc N, exo S). 49 (79%) of the isolates were distributed in three ERIC PCR subgroups and showed 62% of similarity. The remaining 13 strains generated unique patterns.

[Antibiotic susceptibility and occurrence of ESBL, IBL and MBL in Pseudomonas aeruginosa strains].

The aim of this study was to evaluate the drug susceptibility of P. aeruginosa strains and to detect strains producing inducible beta-lactamases (IBL), extended-spectrum beta-lactamases (ESBL), and metallo-beta-lactamases (MBL). During 6 month (October 2005 - March 2006), 66 strains of P.

A comparative evaluation of PCR ribotyping and ERIC PCR for determining the diversity of clinical Pseudomonas aeruginosa isolates.

Two typing methods were evaluated, utilizing 62 clinical strains of Pseudomonas aeruginosa, to assess their usefulness as tools to study the bacterial diversity within this complex group. Genetic diversity was determined by PCR ribotyping and enterobacterial repetitive intergenic consensus (ERIC) PCR. By these methods, 9 and 36 genotypes were found, respectively.

Effect of neuraminidase on adherence of Pseudomonas aeruginosa to human buccal epithelial cells. Inhibition of adhesion by monosaccharides.

The aim of this study was to evaluate the actiion of Clostridium perfringens neuraminidase on the adherence of 28 strains of Pseudomonas aeruginosa which were isolated from humans, different animals and environment to human buccal epithelial cells (BECs). Two reference strainns--NCTC 6749 and ATCC 27853 were also examined. Incubation of cells with the enzyme significantly increased bacterial adherence (a mean number of bacteria adhering to cells amounted 19.

[Inhibition of adhesion to buccal epithelial cells of Pseudomonas aeruginosa by dextran].

Adhesion of Pseudomonas aeruginosa strains to buccal epithelial cells appears to be a necessary precondition for colonization and infection of respiratory tract. There are many strategies to prevent host organisms for Pseudomonas aeruginosa. The purpose of these studies was to evaluate the potential for preventing adhesion of Pseudomonas aeruginosa to epithelial cells with dextran.

Reduction in the adherence of Pseudomonas aeruginosa to human buccal epithelial cells with neuraminidase inhibition.

The aim of this study was to evaluate the reduction in the adherence of 33 strains of Pseudomonas aeruginosa isolated from humans and different animals to human buccal epithelial cells with neuraminidase inhibition. Buccal epithelial cells were incubated with strains of Pseudomonas aeruginosa in the presence or absence of the neuraminidase inhibitors, 2,3-dehydro-2-deoxy-N-acetyl-neuraminic acid (DANA) or N-acetyl-neuraminic acid (NANA). Incubation of cells with bacteria in the presence of either DANA or NANA reduced bacterial adherence significantly by 35.

[Detection of Pseudomonas aeruginosa biofilm on medical biomaterials].

The adhesion and biofilm formation of Pseudomonas aeruginosa strains on the surface of catheters made of various polymers (PU, SL, PCW) were determined in vitro. It was used the method by Richards et al. with modification of Rózalska et al.

Adherence of Pseudomonas aeruginosa to human buccal epithelial cells.

The aim of this study was to evaluate adherence of 83 strains of Pseudomonas aeruginosa isolated from humans and different animals to trypsin-treated buccal cells. We have demonstrated that Pseudomonas aeruginosa attached to trypsin-treated buccal cells in far greater numbers than to cells from controls (normal buccal epithelial cells). The mean number of bacteria adhering to trypsin-treated cells amounted 107.

[The effect of culture conditions on hydrophobic properties of Pseudomonas aeruginosa].

The cell surface hydrophobicity (CSH) plays an important role in a adhesion of bacteria on solid surfaces. CSH of 62 Pseudomonas aeruginosa strains isolated from humans and different animals was assessed using the ammonium sulfate salt aggregation test. Bacteria were grown for 24 h and 48 h at a room temperature (22 degrees C) and 37 degrees C on enrichment broth and agar (Biomed) and tryptic soy agar (Difco).