Exercise Training Decreases Nitrite Concentration in the Heart and Locomotory Muscles of Rats Without Changing the Muscle Nitrate Content.

Background: Skeletal muscles are postulated to be a potent regulator of systemic nitric oxide homeostasis. In this study, we aimed to evaluate the impact of physical training on the heart and skeletal muscle nitric oxide bioavailability (judged on the basis of intramuscular nitrite and nitrate) in rats.

Methods And Results: Rats were trained on a treadmill for 8 weeks, performing mainly endurance running sessions with some sprinting runs.

Effect of synchronization of firings of different motor unit types on the force variability in a model of the rat medial gastrocnemius muscle.

The synchronized firings of active motor units (MUs) increase the oscillations of muscle force, observed as physiological tremor. This study aimed to investigate the effects of synchronizing the firings within three types of MUs (slow-S, fast resistant to fatigue-FR, and fast fatigable-FF) on the muscle force production using a mathematical model of the rat medial gastrocnemius muscle. The model was designed based on the actual proportion and physiological properties of MUs and motoneurons innervating the muscle.

Contractile history affects sag and boost properties of unfused tetanic contractions in human quadriceps muscles.

Purpose: A period of extra-efficient force production ("boost") followed by a decline in force ("sag") is often observed at the onset of unfused tetanic contractions. We tested the hypothesis that in human muscle boost and sag are diminished in repeated contractions separated by short rest periods and are re-established or enhanced following long rest periods.

Methods: Two sets of 3 unfused tetanic contractions were evoked in the right quadriceps muscle group of 29 participants via percutaneous stimulation of the femoral nerve.

Biomechanical conditioning of the motor unit transitory force decrease following a reduction in stimulation rate.

Background: The biomechanical background of the transitory force decrease following a sudden reduction in the stimulation frequency under selected experimental conditions was studied on fast resistant motor units (MUs) of rat medial gastrocnemius in order to better understand the mechanisms of changes in force transmission.

Methods: Firstly, MUs were stimulated with three-phase trains of stimuli (low-high-low frequency pattern) to identify patterns when the strongest force decrease (3-36.5%) following the middle high frequency stimulation was observed.

Initial force production before sag is enhanced by prior contraction followed by a 3-minute rest period in fast motor units of the rat medial gastrocnemius.

Unfused tetanic contractions evoked in fast motor units exhibit extra-efficient force production at the onset of contraction, an effect called "boost". Boost is diminished in subsequent contractions if there is a short rest period between contractions, but can be re-established with a longer period of rest. We tested the hypothesis that contractile activity and rest could enhance boost-related metrics.

Transitory force decrease following a sudden reduction in stimulation frequency in motor units of rat medial gastrocnemius.

Effects of a sudden decrease in the stimulation frequency for motor unit force were studied in rat medial gastrocnemius. For 161 functionally isolated single motor units of three types (S, FR, FF), unfused tetanic contractions were evoked by three-phase trains of stimuli (low-high-low frequency). The course of the tetanus at the onset of the third phase of the force recording was analyzed in tetani with variable fusion degree.

Adaptation of motor unit contractile properties in rat medial gastrocnemius to treadmill endurance training: Relationship to muscle mitochondrial biogenesis.

This study aimed at investigating the effects of 2, 4 and 8 weeks of endurance training on the contractile properties of slow (S), fast fatigue resistant (FR) and fast fatigable (FF) motor units (MUs) in rat medial gastrocnemius (MG) in relation to the changes in muscle mitochondrial biogenesis. The properties of functionally isolated MUs were examined in vivo. Mitochondrial biogenesis was judged based on the changes in mitochondrial DNA copy number (mtDNA), the content of the electron transport chain (ETC) proteins and PGC-1α in the MG.

Force regulation and electrical properties of motor units in overloaded muscle.

Introduction: The influence of long-term muscle overload on force regulation and electrical properties of motor units (MUs) was investigated in rats.

Methods: Compensatory overload of the medial gastrocnemius was induced by tenotomy of its synergists. Electrophysiological experiments were performed on functionally isolated MUs 3 months after the surgery.

Changes in motor unit properties in SOD1 (G93A) rats.

Introduction: In amyotrophic lateral sclerosis (ALS), progressive death of motor neurons results in denervation and reinnervation of muscles. It is not clear how ALS affects the properties of motor units (MUs).

Methods: Properties of single MUs in the medial gastrocnemius (MG) muscle of rats bearing the human mutated superoxide dismutase gene type 1 (SOD1) were determined at 3 stages: asymptomatic (ALS I); early symptomatic (ALS II); and terminal (ALS III).

Time-related changes of motor unit properties in the rat medial gastrocnemius muscle after the spinal cord injury. II. Effects of a spinal cord hemisection.

The contractile properties of motor units (MUs) were investigated in the medial gastrocnemius (MG) muscle in rats after the spinal cord hemisection at a low thoracic level. Hemisected animals were divided into 4 groups: 14, 30, 90 and 180 days after injury. Intact rats formed a control group.