Immunochemical studies and gene cluster relationships of closely related O-antigens of Aeromonas hydrophila Pt679, Aeromonas popoffii A4, and Aeromonas sobria K928 strains classified into the PGO1 serogroup dominant in Polish aquaculture of carp and rainbow trout.

The present study included three Aeromonas sp. strains isolated from fish tissues during Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreaks on commercial farms, i.e.

Structure and gene cluster annotation of the O-antigen of Aeromonas sobria strain K928 isolated from common carp and classified into the new Aeromonas PGO1 serogroup.

Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup. The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and H and C NMR spectroscopy.

Structure of the lipopolysaccharide O-antigen of Aeromonas encheleia strain A4 representing the new PGO1 serogroup of aeromonads prevailing in Polish aquaculture.

The structure of the O-specific polysaccharide (OPS) from Aeromonas encheleia strain A4 lipopolysaccharide was investigated. A. encheleia strain A4, classified into the new provisional serogroup PGO1 predominating among aeromonads in Polish aquaculture, was isolated from common carp tissues during an outbreak of MAI/MAS disease on a fish farm.

Structural Studies of the Lipopolysaccharide of bv. Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms.

In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of bv. strain K133, which was isolated from the kidney of carp ( L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm.

Structure of the disaccharide repeating unit of O-specific polysaccharide isolated from Aeromonas veronii strain Bs8 pathogenic to common carp (Cyprinus carpio).

The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas veronii strain Bs8, which is pathogenic to common carp (Cyprinus carpio), after mild acid hydrolysis followed by gel-permeation chromatography. The high-molecular-mass OPS fraction was investigated using chemical methods, mass spectrometry, and H and C NMR spectroscopy techniques, including 2D homonuclear H,H TOCSY, DQF COSY, NOESY, and heteronuclear H-detected H,C HSQC, and HMBC experiments. The analysis revealed that the O-specific polysaccharide contains sugars with the galacto configuration of the ring and is composed of a disaccharide repeating unit with the following structure.

Structure of the O-specific polysaccharide of Aeromonas veronii bv. sobria strain Pt393 isolated from rainbow trout (Oncorhynchus mykiss), which contains a rarely occurring sugar 4-acetamido-4,6-dideoxy-d-galactose, tomosamine.

The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas veronii bv. sobria strain Pt393, which is pathogenic to the rainbow trout (Oncorhynchus mykiss), after mild acid hydrolysis followed by GPC. The high-molecular-weight OPS fraction was studied with chemical methods, mass spectrometry, and H and C NMR spectroscopy techniques, including 2D H,H COSY, TOCSY, NOESY, H-detected heteronuclear H,C HSQC, and HMBC experiments.

Structural and Serological Studies of the O6-Related Antigen of bv. Strain K557 Isolated from on a Polish Fish Farm, which Contains L-perosamine (4-amino-4,6-dideoxy-L-mannose), a Unique Sugar Characteristic for Serogroup O6.

Amongst spp. strains that are pathogenic to fish in Polish aquacultures, serogroup O6 was one of the five most commonly identified immunotypes especially among carp isolates. Here, we report immunochemical studies of the lipopolysaccharide (LPS) including the O-specific polysaccharide (O-antigen) of bv.

A Unique Sugar l-Perosamine (4-Amino-4,6-dideoxy-l-mannose) Is a Compound Building Two O-Chain Polysaccharides in the Lipopolysaccharide of Strain JCM 3968, Serogroup O6.

Lipopolysaccharide (LPS) is the major glycolipid and virulence factor of Gram-negative bacteria, including spp. The O-specific polysaccharide (O-PS, O-chain, O-antigen), i.e.

A Mutation in the Gene Alters the Physicochemical Properties of the Bacterial Cell Wall and Reduces Survival inside .

In our previous report, we had shown that the free-living amoeba influenced the abundance, competiveness, and virulence of NZP2213, the microsymbiont of agriculturally important plants of the genus . The molecular basis of this phenomenon; however, had not been explored. In the present study, we demonstrated that , the -acetyltransferase encoding gene located in the lipopolysaccharide (LPS) synthesis cluster of , was responsible for maintaining the protective capacity of the bacterial cell envelope, necessary for the bacteria to fight environmental stress and survive inside amoeba cells.