'Like we definitely have to go greener, but…': Analysing affective-discursive practices in populist environmental discourse.

Previous studies on environmental issues in right-wing populism have mostly focused on political actors and their argumentation. In contrast, this study examines environmental populist discourse from the perspective of laypeople in Finland. We used interviews (n = 25) to analyse affective-discursive practices in environmental talk, identifying four partly interrelated practices: belittling the 'annoying liberals', constructing the ordinary rural people as victims, externalizing blame to the 'real' polluters, and glorifying Finnish nature.

Red-pilled mama bears and enlightened power goddesses: Discursive constructions of feminine identities in a conspiracy theory space.

Previous research into the gendered social identity work involved in conspiracy theories (CTs) has largely focused on expressions of masculinity. The present study investigates the employment and mobilization of feminine identities in online Covid-19 conspiracy theory seminars through a critical discursive psychological perspective. The analysis finds three interpretative repertoires for representing the pandemic: the totalitarianism repertoire, the corrupt medical profession repertoire and the awakening repertoire.

'You truly are the worst kind of racist!': Argumentation and polarization in online discussions around gender and radical-right populism.

The role of women in populist and radical right-wing parties is a topic that has gained increased scholarly attention. The aim of this article is to add to this literature by analysing how a female right-wing populist leader becomes positioned in online interactions in the hybrid media system. In doing so, the study seeks to make a twofold contribution to research on populist and radical right discourse online: to explore the ways in which notions related to gender and radical-right populism are constructed in such discourse and to shed light on their argumentative character.

Managing stake and accountability in Prime Ministers' accounts of the "refugee crisis": A longitudinal analysis.

Taking a (critical) discursive psychological approach, the present study explores the identity management of the Finnish and Swedish Prime Ministers (PM) in relation to the "refugee crisis" and their countries' asylum policies. By taking a longitudinal approach and analysing the PMs' accounts of the "refugee crisis" from 1-year period, we focused on the ways rhetorical devices related to ethos, logos, and pathos were used to manage the issues of stake and accountability, as well as on the ways in which categories were worked up to serve particular functions. Our comparative analysis demonstrated significant similarities in the Finnish and Swedish PMs' talk, especially with regard to the transfer from a discourse of pathos and ethos, describing refugees in terms of individualism and humaneness, to a discourse of logos, emphasizing rationality, justifying sharpened immigration policies, and homogenizing refugees.

The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo.

Retinoic acid receptor (RAR) and retinoid X receptor are ligand-induced transcription factors that belong to the nuclear receptor family. The receptors are activated by small hydrophobic compounds, such as all-trans-retinoic acid and 9-cis-retinoic acid, respectively. Interestingly, these receptors are also targets for a number of exogenous compounds.

High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week.

The demand for high-throughput recombinant protein production has markedly increased with the increased activity in the field of proteomics. Within the Human Protein Atlas project recombinantly produced human protein fragments are used for antibody production. Here we describe how the protein expression and purification protocol has been optimized in the project to allow for handling of nearly 300 different proteins per week.

An amphioxus orthologue of the estrogen receptor that does not bind estradiol: insights into estrogen receptor evolution.

Background: The origin of nuclear receptors (NRs) and the question whether the ancestral NR was a liganded or an unliganded transcription factor has been recently debated. To obtain insight into the evolution of the ligand binding ability of estrogen receptors (ER), we comparatively characterized the ER from the protochordate amphioxus (Branchiostoma floridae), and the ER from lamprey (Petromyzon marinus), a basal vertebrate.

Results: Extensive phylogenetic studies as well as signature analysis allowed us to confirm that the amphioxus ER (amphiER) and the lamprey ER (lampER) belong to the ER group.

3-Methylcholanthrene displays dual effects on estrogen receptor (ER) alpha and ER beta signaling in a cell-type specific fashion.

The biological effects of 17beta-estradiol (E(2)) are mediated by the two estrogen receptor (ER) isoforms ERalpha and ERbeta. These receptors are ligand-inducible transcription factors that belong to the nuclear receptor superfamily. These receptors are also targets for a broad range of natural and synthetic compounds that induce ER activity, including dietary compounds, pharmaceuticals, and various types of environmental pollutants such as bisphenols and polychlorinated hydroxy-biphenyls.

The transcription factor aryl hydrocarbon receptor nuclear translocator functions as an estrogen receptor beta-selective coactivator, and its recruitment to alternative pathways mediates antiestrogenic effects of dioxin.

The biological effects of dioxins are mediated by the aryl hydrocarbon receptor (AhR) and its dimerization partner, the AhR nuclear translocator (ARNT), and include interference with hormonal signaling pathways like the response to estrogens. The effects of estrogens are mediated by two estrogen receptor (ER) isoforms, ERalpha and ERbeta, which belong to the family of nuclear receptors. We have previously shown that ARNT can act as coactivator of the ERs.

Uterine rupture and perinatal outcome.

Background: In view of the increasing number of caesarean sections (CS), we wanted to investigate the clinical aspects of uterine rupture including perinatal outcome.

Methods: A retrospective investigation of 24,181 deliveries at Stockholm South General Hospital between 1999 and 2004. Patient notes from cases with ICD-codes 0710 and 0711 were studied together with charts from previous deliveries and neonatal data from the Paediatric Department.

Diet-derived polyphenol metabolite enterolactone is a tissue-specific estrogen receptor activator.

Numerous dietary compounds can modify gene expression by binding to the members of the nuclear receptor superfamily of transcription factors. For example, dietary polyphenols, such as soy isoflavones genistein and daidzein, modulate the activity of the estrogen receptors (ERs)-alpha and ERbeta. An additional class of dietary polyphenols that modulate cellular signaling pathways are lignans, compounds that are common constituents of Western diets.

Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.

Unliganded thyroid hormone receptors (apoTRs) repress transcription of hormone-activated genes by recruiting corepressors to the promoters. In contrast, on promoters containing so-called negative thyroid hormone response elements (nTREs), apoTRs activate transcription. A number of different molecular mechanisms have been described as to how apoTRs activate transcription varying with the target gene of the study.

The Ah receptor inhibits estrogen-induced estrogen receptor beta in breast cancer cells.

We have studied the effect of the aryl hydrocarbon receptor ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on estrogen receptor (ER) beta gene expression in the human breast cancer cell line, T47D. TCDD inhibited 17beta-estradiol (E2)-induced up-regulation of both ER beta wild type and ER beta cx mRNA. Cycloheximide pre-treatment had no inhibitory effect, and the estimated half-life of ER beta mRNA of about 33 min was not changed by any hormone administration.

Regulation of postnatal lung development and homeostasis by estrogen receptor beta.

Estrogens have well-documented effects on lung development and physiology. However, the classical estrogen receptor alpha (ERalpha) is undetectable in the lung, and this has left many unanswered questions about the mechanism of estrogen action in this organ. Here we show, both in vivo and in vitro, that ERbeta is abundantly expressed and biologically active in the lung.

The basic helix-loop-helix-PAS protein ARNT functions as a potent coactivator of estrogen receptor-dependent transcription.

The biological effects of estrogens are mediated by the estrogen receptors ERalpha and ERbeta. These receptors regulate gene expression through binding to DNA enhancer elements and subsequently recruiting factors such as coactivators that modulate their transcriptional activity. Here we show that ARNT (aryl hydrocarbon receptor nuclear translocator), the obligatory heterodimerization partner for the aryl hydrocarbon receptor and hypoxia inducible factor 1alpha, functions as a potent coactivator of ERalpha- and ERbeta- dependent transcription.