Publications by authors named "Katarina Janikova"
Identifying novel epigenetic biomarkers is a promising way to improve the clinical management of patients with breast cancer. Our study aimed to determine the methylation pattern of 25 tumor suppressor genes (TSG) and select the best methylation biomarker associated with clinicopathological features in the cohort of Slovak patients diagnosed with invasive ductal carcinoma (IDC). Overall, 166 formalin-fixed, paraffin-embedded (FFPE) tissues obtained from patients with IDC were included in the study.
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- PARP inhibitors work by blocking the repair of single-strand DNA breaks caused by tumor growth, leading to cell death when the homologous recombination repair system is dysfunctional, especially relevant for patients with serous ovarian epithelial tumors.
- Studies show that women with BRCA mutations exhibit the highest progression-free survival rates, although PARP inhibitors are also beneficial for those with a disrupted homologous recombination deficiency system, regardless of BRCA status.
- The research analyzed a group of women with high-grade serous ovarian cancer who had undergone at least two chemotherapy treatments, using the SOPHIA DDM™ tool to identify pathogenic variants in BRCA genes, confirming mutations in 50% of the participants in order to enhance patient management and treatment
Article Synopsis
- The Ministry of Health in Slovakia created a genomics surveillance workgroup to monitor SARS-CoV-2 using whole-genome sequencing (WGS) during the pandemic.
- A total of 33,024 SARS-CoV-2 isolates were sequenced from March 2021 to March 2022, with the majority successfully deposited in the GISAID database.
- The study identified four variants of concern (Alpha, Beta, Delta, and Omicron) and documented their prevalence during three major waves of infections in Slovakia.
Article Synopsis
- - The incidence of colorectal cancer (CRC) has increased significantly over the last decade, leading to a focus on understanding the disease's pathology and treatment resistance, particularly linked to cancer stem cells.
- - A study in Slovakia aimed to examine specific stem cell markers (CD133, CD166, and Lgr5) in CRC tumors using flow cytometry, as well as analyze DNA methylation of CRC biomarkers in patient samples.
- - Results indicated that metastatic tumors had higher CD133 cell levels than primary tumors, with a correlation to CRC aggressiveness and lymphatic invasion, highlighting CD133's potential role as a key marker in CRC pathology.
The global pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is having a tremendous impact on the global economy, health care systems and the lives of almost all people in the world. The Central European country of Slovakia reached one of the highest daily mortality rates per 100,000 inhabitants in the first 3 months of 2021, despite implementing strong prophylactic measures, lockdowns and repeated nationwide antigen testing. The present study reports a comparison of the performance of the Standard Q COVID-19 antigen test (SD Biosensor) with three commercial RT-qPCR kits (vDetect COVID-19-MultiplexDX, gb SARS-CoV-2 Multiplex-GENERI BIOTECH Ltd.
View Article and Find Full Text PDFOvarian cancer is the leading cause of mortality among all gynecological cancers in developed countries and its most common and most lethal type is the high-grade serous ovarian carcinoma (HGSC). At the molecular level, nearly half of all HGSCs exhibit ineffective homologous DNA recombination and disruption of DNA damage/repair pathway inactivation caused often by BRCA1 and BRCA2 gene mutation. Recently, the detection of BRCA1/2 mutations became important for personalized treatment of HGSC patients with the PARP-inhibitors in the defined clinical setting of relapse after positive adjuvant platinum-based chemotherapeutic response.
View Article and Find Full Text PDFNatural substances of plant origin exert health beneficiary efficacy due to the content of various phytochemicals. Significant anticancer abilities of natural compounds are mediated via various processes such as regulation of a cell's epigenome. The potential antineoplastic activity of plant natural substances mediated by their action on posttranslational histone modifications (PHMs) is currently a highly evaluated area of cancer research.
View Article and Find Full Text PDFThe development of the new technologies such as the next-generation sequencing (NGS) makes more accessible the diagnosis of genetically heterogeneous diseases such as Lynch syndrome (LS). LS is one of the most common hereditary form of colorectal cancer. This autosomal dominant inherited disorder is caused by deleterious germline mutations in one of the mismatch repair (MMR) genes - MLH1, MSH2, MSH6 or PMS2, or the deletion in the EPCAM gene.
View Article and Find Full Text PDFThe aim of the study was to establish the frequency of hypovitaminosis D in children with type 1 diabetes mellitus (T1D), its influence on biochemical and densitometric parameters and the relation to diabetic nephropathy. 58 children with T1D at the age 9-19 years were enrolled to the study. Vitamin D concentration less than 30 ng/ml was considered as insufficient.
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