Effect of withholding food versus feeding on creatinine, symmetric dimethylarginine, cholesterol, triglycerides, and other biochemical analytes in 100 healthy dogs.

Background: Withholding food is often recommended before collection of blood for routine biochemical analysis in dogs despite a paucity of evidence to support this requirement.

Objectives: To compare measurements of selected biochemical analytes collected before and after feeding in clinically healthy dogs.

Animals: One hundred clinically healthy staff- and student-owned dogs weighing ≥15 kg.