Involvement of miR-199a-5p-loaded mesoporous silica nanoparticle-polyethyleneimine-KALA in osteogenic differentiation.

Background/purpose: While there are numerous reports on surgical techniques and materials for bone grafting, limited methods are available to enhance the body's inherent capacity to heal bones. Here we investigated microRNA-199a (miR-199a), a molecular that promotes osteoblast differentiation and bone healing.

Materials And Methods: To construct a miR-199a delivery complex, miR-199a-5p mimics were coated with mesoporous silica nanoparticles (MSNs) following modified with polyethyleneimine (PEI) and peptide WEAKLAKALAKALAKHLAKALAKALKACEA (KALA) to obtain 199a-5p-loaded MSN-PEI-KALA.

Evaluation of a hydroxyapatite-crosslinked fish gelatin membranes.

Background/purpose: Porcine collagen is widely used in regenerative therapies to generate membranes for bone augmentation. However, porcine or bovine gelatin or collagen is often not appropriate for patients with creed and religious beliefs or for allergic reasons. In this study, we evaluated the potential of fish gelatin to generate membranes.

A modal analysis of implant-supported overdentures installed on differently positioned sets of dental implants.

This study evaluated the three vibration characteristics, namely, natural frequency, damping ratio, and natural mode, together with maximum displacement of a two-implant-supported overdenture (IOD) at different locator attachment positions using experimental modal analysis (EMA). Edentulous mandibular models with a gingival thickness of 1 mm or 3 mm were prepared, into which dental implants were placed using a fully guided surgical template designed with simulation software, the locator abutments were fastened, and the IODs were then fabricated. The implant positions were bilaterally marked at the lateral incisor, first premolar, and first molar regions.

Correction: Lu et al. Periodontal Pathogen Adhesion, Cytotoxicity, and Surface Free Energy of Different Materials for an Implant Prosthesis Screw Access Hole. 2022, , 329.

In the original publication [...

An In Vitro Evaluation of Selenium Nanoparticles on Osteoblastic Differentiation and Antimicrobial Properties against .

Despite numerous treatment methods, there is no gold standard for the treatment of peri-implantitis-an infectious peri-implant disease. Here, we examined selenium nanoparticles (SeNPs) at a wide range of concentrations to investigate their cytotoxicity, regulation of osteoblastic differentiation, and assessed the antibacterial effect against . SeNPs (mean size: 70 nm; shape: near-spherical; concentration: 0-2048 ppm) were tested against the MC3T3-E1 osteoblast precursor cell line and red complex pathogen.

Microbiological Comparison of Different Sealing Materials for the Access Holes of Implant Restorations.

Purpose: To evaluate the performance of sealing materials used in the screw-access holes of screw-retained implant final superstructures in vivo and in vitro.

Materials And Methods: Twenty-one screw-access holes in the final superstructures were randomly divided into three groups (each group, n = 7). Following disinfection and isolation, all access holes were initially filled with sterilised cotton pellets of the same weight.

Periodontal Pathogen Adhesion, Cytotoxicity, and Surface Free Energy of Different Materials for an Implant Prosthesis Screw Access Hole.

: Oral implant restorations are an excellent treatment option for edentulous patients; however, periodontopathogenic bacteria have been found in the microgaps between implant-abutment junctions. Implant designs to limit the microgaps have been extensively studied. However, studies have shown microgaps continue to exist, allowing for the leakage of bacteria into the implant system.

Impact of Bone Augmentation of Facial Bone Defect around Osseointegrated Implant: A Three Dimensional Finite Element Analysis.

(1) Background: When dental implants are placed at the esthetic zone, facial bone fenestration might be expected. This study aimed to evaluate the biomechanical effect of bone augmentation around implants with facial bone fenestration defects using the finite element method. (2) Methods: An anterior maxillary region model with facial concavity was constructed with a threaded implant inserted following the root direction, resulting in apical threads exposure to represent the fenestration model.

Fluctuation of salivary alpha-amylase activity levels and vital signs during dental implant surgery.

Background: Salivary alpha-amylase (sAA) activity level is thought to be an indicator of mental stress. However, the relationship between sAA activity levels and mental stress in patients during dental implant treatment has not been studied. In the present study, we aimed to examine the correlation between sAA activity levels and changes in patients' vital signs during dental implant surgery.

Patient-specific establishment of bacterial composition within the peri-implant microbiota during the earliest weeks after implant uncovering.

Background And Objective: Dysbiosis, a loss of balance in the microbiota, is a potential factor of peri-implantitis. However, compositional change of the peri-implant microbiota soon after implant uncovering is still unknown. In this study, bacterial composition in the peri-implant sulcus was examined to understand the establishment of bacterial composition within the peri-implant microbiota during the earliest weeks after implant uncovering.

Efficacy of a novel membrane comprising a copolymer of L-lactic acid and glycolic acid in osteoblasts in vitro.

In guided bone regeneration (GBR), a barrier membrane is applied to provide a space for bone regeneration to promote cell adhesion, cell proliferation, and bone regeneration by inducing cell migration to the surface of the bone-deficient site. We prepared a non-woven absorbable membrane for GBR comprised of a copolymer of L-lactic acid and glycolic acid (PLLGA) using the dry spinning method. A thin and dense membrane comprising unsintered hydroxyapatite (uHA) and poly-L-lactic acid (PLLA) was used for comparison.

Influence of Age on Calvarial Critical Size Defect Dimensions: A Radiographic and Histological Study.

Calvarial critical-size defect has been used to assess techniques and materials in the bone regeneration field. Previous studies utilized young adult rats with 3 months of age, which might not reflect the geriatric conditions. This study aimed to assess the dimensions of the calvarial critical-size defect in aged rats.

Evaluation of a Cetylpyridinium Chloride-, Dipotassium Glycyrrhizinate-, and Tranexamic Acid-based Mouthwash after Implant Placement: A Double-blind Randomised Clinical Trial.

Purpose: To evaluate the positive effects of a CPC-, GK2-, and TXA-based (CPC/GK2/TXA) mouthwash after implant placement.

Materials And Methods: Twenty patients (n = 20) who underwent posterior implant-placement surgery were randomly and evenly allocated to the study or the placebo group. After the mouthwash was used 3x/day for 7 to 10 days postoperatively, sutures were analysed by counting the colony-forming units (CFU) for total aerobes, total G [-] anaerobes, total enterobacteria and total H.

Autologous micrografts from the palatal mucosa for bone regeneration in calvarial defects in rats: a radiological and histological analysis.

Background: The application of dental implants is often restricted by bone volume. In such cases, bone grafts are required, although bone graft materials have some disadvantages. Therefore, other effective approaches are needed.

Discriminating Microbial Community Structure Between Peri-Implantitis and Periodontitis With Integrated Metagenomic, Metatranscriptomic, and Network Analysis.

Peri-implantitis and periodontitis are both polymicrobial diseases induced by subgingival plaque accumulation, with some differing clinical features. Studies on the microbial and gene transcription activity of peri-implantitis microbiota are limited. This study aimed to verify the hypothesis that disease-specific microbial and gene transcription activity lead to disease-specific clinical features, using an integrated metagenomic, metatranscriptomic, and network analysis.

Investigation of different electrochemical cleaning methods on contaminated healing abutments in vitro: an approach for metal surface decontamination.

Background: To evaluate the effects of electrolysis on cleaning the contaminated healing abutment surface and to detect the optimal condition for cleaning the contaminated healing abutment.

Methods: Ninety healing abutments removed from patients were placed in 1% sodium dodecyl sulfate solution and randomly divided for electrolysis with 7.5% sodium bicarbonate in the following three different apparatuses (N = 30): two stainless steel electrodes (group I), a copper electrode and a carbon electrode (group II), and two carbon electrodes (group III).

Comparison of splinted and non-splinted superstructures of three implants placed in a mandibular distal extension model with missing teeth using modal analysis.

This study evaluated the effects of two types of superstructures (splinted crown and non-splinted crown) on four vibration characteristics (natural frequency, damping ratio (DAR), vectors in antiphase, maximum displacement (MDP)) by using modal analysis. These structures were fabricated on three implants placed in the mandibular free-end defect model. After placing three implants on a mandibular distal extension model with missing teeth, the three-unit splinted and non-splinted crowns were designed on the CAD software.

Evaluation of maxillary sinus floor augmentation with the crestal approach and beta-tricalcium phosphate: a cone-beam computed tomography 3- to 9-year follow-up.

Background: We performed maxillary sinus floor augmentation using the crestal approach and beta-tricalcium phosphate (β-TCP) and a long-term evaluation using cone-beam computed tomography (CBCT).

Methods: Twenty-three patients (30 implants) underwent sinus floor augmentation using the osteotome technique. Subjects underwent CBCT imaging before surgery, immediately after surgery, and at follow-up (≥ 3 years after surgery).

Drinking green tea alleviates alveolar bone resorption in ligature-induced periodontitis in mice.

Objectives: It has been reported that green tea exerts antibacterial, anti-inflammatory, and antioxidant effects. The purpose of the present study was to evaluate the effects of drinking green tea on bone resorption in ligature-induced periodontitis in mice.

Methods: Sixty C57BL/6 eight-week-old male mice were used.

Evaluation of residual contamination on healing abutments after cleaning with a protein-denaturing agent and detergent.

Objective: This study evaluated the cleaning potential of a protein-denaturing agent with or without anionic detergent by monitoring the residual contamination on healing abutments used for dental implant treatment.

Method And Materials: Forty contaminated healing abutments removed from patients were randomized and immediately treated with differing cleaning methods; either Method A (presoaking in 1% sodium dodecyl sulfate followed by ultrasonication with 4 mol/L guanidine hydrochloride), or Method B (soaking in distilled water followed by ultrasonication with 4 mol/L guanidine hydrochloride) was used. Samples were stained with phloxine B and photographed using a light microscope.

Enhancement of mineralization on porous titanium surface by filling with nano-hydroxyapatite particles fabricated with a vacuum spray method.

Nano-hydroxyapatite (nano-HA) coating has been proved to be effective to modify the titanium surface for better bone formation. A simple and economical nano-HA coating method that filling the nano-HA particles into the porous titanium substrate by using vacuum suction was introduced in this study. Two kinds of nano-HA modified titanium surfaces, nano-HA filled type (nHA-f) and nano-HA coated type (nHA-c), were prepared by a 2-step and 3-step protocol, respectively.

Combined in silico analysis identified a putative tooth root formation-related gene, Chd3, which regulates DNA synthesis in HERS01a cells.

There exists a close connection between changes occurring in the teeth and those occurring in the jaw during the evolutionary process. In mammals, the roots of teeth are supported, along with periodontal ligaments and alveolar bones by a unique structure termed the gomphosis. In the present study, we performed combined in silico analysis using the information obtained from various DNA microarrays and identified 19 putative tooth root formation-related genes.

Application of low-crystalline carbonate apatite granules in 2-stage sinus floor augmentation: a prospective clinical trial and histomorphometric evaluation.

Purpose: The purpose of this study was to elucidate the efficacy and safety of carbonate apatite (COAp) granules in 2-stage sinus floor augmentation through the radiographic and histomorphometric assessment of bone biopsy specimens.

Methods: Two-stage sinus floor augmentation was performed on 13 patients with a total of 17 implants. Radiographic assessment using panoramic radiographs was performed immediately after augmentation and was also performed 2 additional times, at 7±2 months and 18±2 months post-augmentation, respectively.