Stability-indicating HPLC assay for lysine-proline-valine (KPV) in aqueous solutions and skin homogenates.

A simple, sensitive and stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for a bioactive peptide, lysine-proline-valine (KPV) in aqueous solutions and skin homogenates. Chromatographic separation was achieved on a reversed phase Phenomenex C18 column (4.6 × 250 mm, packed with 5 µm silica particles) with a gradient mobile phase consisting of 0.

Lipid materials for topical and transdermal delivery of nanoemulsions.

Over the past two decades, nanoemulsions have gained significant scientific attention because of their unique features such as high solubilization capacity, spontaneous formation, enhanced thermodynamic stability, ability to load both hydrophilic and hydrophobic drug molecules, enhanced stability of the encapsulated therapeutic molecule, and high diffusion/absorption rates. Further, they have applications in dermal and epidermal targeting for various skin disorders. The materials used in nanoemulsion formulations can greatly influence the in vitro and in vivo performance of the therapeutic moiety.

Effect of lipophilicity on microneedle-mediated iontophoretic transdermal delivery across human skin in vitro.

The effect of lipophilicity of drug on the microneedle (MN)-mediated iontophoretic delivery across dermatomed human skin was studied. Beta blockers with similar pKa but varied log P values were selected as model drugs in this study. Iontophoresis (ITP) or MNs, when used independently, increased the transdermal flux of beta blockers as compared with passive delivery (PD).

Polymeric and lipid-based materials for topical nanoparticle delivery systems.

The delivery of drugs and cosmetic actives to the skin by nanoparticle formulations has a number of advantages over conventional formulations. Nanoparticle formulations offer protection of incorporated active compounds against chemical degradation, more flexibility in modulating the release of the compound, the use of well-tolerated excipients, and feasibility of large scale production. The materials used in the nanoparticle synthesis and formulation can influence product stability, active ingredient properties and delivery to the intended site of action.