Publications by authors named "Kasturi Goswami"
Article Synopsis
- The study investigates the MCM helicase from the archaeon Picrophilus torridus, highlighting its unique activity and interactions.
- Although PtMCM can hydrolyze ATP, it struggles to unwind DNA on its own, with activity only observed in acidic conditions when partnered with PtGINS.
- This research is significant as it represents the first time an MCM helicase has been shown to perform DNA unwinding strictly at low pH, suggesting diverse mechanisms of replication regulation in archaea that warrant further exploration.
Article Synopsis
- Eukaryotic DNA replication begins with the formation of prereplication complexes (pre-RCs) during the G1 phase to prepare for DNA synthesis in the S phase, involving a complex called the origin recognition complex (ORC) made up of six components (Orc1-6).
- In archaea, the DNA replication process is simpler and involves a single protein that combines functions of both eukaryotic Cdc6 and Orc1, and studies on the acidophile Picrophilus torridus show it has one such combined protein (PtOrc1/Cdc6) that binds to specific DNA sequences and interacts with PCNA.
- The interaction between PtOrc1/Cdc6 and PCNA not
Protein kinase G (PknG) in Mycobacterium tuberculosis has been shown to modulate phagosome-lysosome fusion. The protein has three distinct domains, an N-terminal Trx domain, a kinase domain, and a C-terminal TPR domain. The present study extensively analyzes the roles of these domains in regulating PknG kinase activity and function.
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