Publications by authors named "Kasia Dinkeloo"

Article Synopsis
  • Translating ribosome affinity purification (TRAP) is a method using transgenic plants to isolate ribosomes and the mRNAs they're translating, enabling the study of the translatome through techniques like quantitative PCR or RNA sequencing.
  • A new TRAP system introduces two transgenes: one that constantly produces a ribosomal protein merged with a split green fluorescent protein (GFP) and another that uses a condition-specific promoter to produce a second GFP fragment with an affinity tag, enhancing sensitivity in analysis.
  • This innovative approach allows for better tracking of ribosome activity in plants, especially in scenarios where promoter activity is weak or when studying transient cell types, such as during plant-pathogen interactions.
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Background: Taraxacum officinale, or the common dandelion, is a widespread perennial species recognized worldwide as a common lawn and garden weed. Common dandelion is also cultivated for use in teas, as edible greens, and for use in traditional medicine. It produces latex and is closely related to the Russian dandelion, T.

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Upon infection, plant pathogens become dependent on their hosts for nutrition. Therefore, the interaction between the two organisms is tightly linked to the availability and flux of nutrients in the plant. The plant's nitrogen metabolism is reprogrammed during pathogen attack, likely reflecting plant's response to invasion by the pathogen and active modification by the pathogen to promote feeding.

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Amino acids are essential components of plant metabolism, not only as constituents of proteins, but also as precursors of important secondary metabolites and as carriers of organic nitrogen between the organs of the plant. Transport across intracellular membranes and translocation of amino acids within the plant is mediated by membrane amino acid transporters. The past few years have seen the identification of a new family of amino acid transporters in Arabidopsis, the characterization of intracellular amino acid transporters, and the discovery of new roles for already known proteins.

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