[Enzymes of cyclic nucleotide degradation in eye tissues during experimental ocular herpes].

Activity of phosphodiesterases disintegrating cAMP and cGMP in the cornea, sclera and ciliary body was investigated in health and in different stages of experimental herpetic keratitis. The problems concerning the role of the cyclase system in the pathogenesis of herpetic keratitis and the possibility of applying some of the drugs to the disease treatment are discussed.

[Effect of the water-soluble antioxidant hydroxypyridine-6 on the intensity of lipid peroxidation in eye tissues in experimental ophthalmic herpes].

Lipid peroxidation (LPO) in eye tissues of rabbits with experimental herpetic keratitis was evaluated from the malonic dialdehyde content and chemiluminescence intensity. It appeared that during the critical phase LPO sharply increased in all the tissues under study and in anterior chamber humor. After administration of the synthetic water-soluble antioxidant, hydroxypyridine-6 the subconjunctival content of malonic dialdehyde and chemiluminescence intensity approached normal values accompanied by positive therapeutic effect.

[Main proteolytic inhibitors in ocular herpes].

Alterations in content of main inhibitors of proteolysis alpha 1-antitrypsin and alpha 2-macroglobulin as well as activity of neutral proteases in blood serum were studied in herpetic impairment of human and rabbit retina. Unspecific protective reaction of the blood proteolytic inhibitors developed in ophthalmoherpes. In the acute period of the disease content of alpha 1-antitrypsin was increased and the content of alpha 2-macroglobulin was decreased.

[Corneal lysosomes in experimental ophthalmoherpes].

Activity and intracellular distribution of lysosomal glucosidases (beta-glucosidase and beta-galactosidase) of the cornea as well as membrane permeability were studied in experimental herpetic keratitis. It was demonstrated that the action of herpes simplex entailed labilization of the lysosomal membranes of the cornea together with the decreased strength of lysosomal association with the enzymes thereby favouring the increased enzyme-substrate contact and excess destruction of cellular and exocellular glycosaminoglycans. The data obtained suggest that lysosomes participate in the pathogenesis of herpetic keratitis.

[Incorporation of labeled cortisol into different types of connective tissue].

The properties of early (BFUen) and late (CFUen) erythropoietin-independent progenitors were studied in bone marrow plasma clot cultures of mice. Syngeneic serum was used as a stimulant of colony formation. It was revealed that serum from polycytemic mice did not alter the efficacy of cloning the erythroid progenitors.

[Lipoperoxidation in eye tissues exposed to hormones].

The effect of hormones of varied action spectrum on lipoperoxidation of unsaturated fatty acids in the eye tissues has been studied. Both antioxidant and prooxidant action of diverse hormones on the sclera, cornea and ciliary body has been demonstrated with respect to the hormonal dose, exposure time and other factors. Potential participation of lipoperoxidation processes in the development of ophthalmopatholody is discussed.

[Responses of different types of connective tissue to hormone administration].

The lysosomal glycosidase activity of the eye tissues (the sclera and cornea), the bone tissues and cartilage were studied. The intraperitoneal injection of tyrocalcitonine (TCT), deoxycorticosterone (DOCS), hydrocortisone (HC), and somatotropic hormone (STH) influenced both the activity of beta-galactosidase, beta-glucosidase, and hyaluronidase, the the functional state of thy lysosomal membranes of the connective tissues under investigation. GC and STH caused stabilization, whereas DOCS and large doses of TCT--a labilizing effect on the lysosomal membranes and tissues understudy.

[Distribution of labeled cortisol in the tissues and media of the eye].

Incorporation of cortizol-3H and its uptake by the tissues and media of the eye was studied to expose the target tissue for the given hormone. Cortizol distribution was investigated in the sclera, ciliary body, cornea, iris, the lens capsule, aqeous humour, and the vitreous body. It was shown that the eye tissues and media had different intensity of cortizol incorporation, as well as different character of its elimination.

[Effect of prostaglandin F2alpha on the lysosomal membranes of eye tissues].

It was demonstrated that both in vitro and in intravenous injection of prostaglandin F2alpha there occurred labilization of the membranes of sclera and ciliary body lysosomes, in difference from the cornea in rabbits. Glycosidase activity appeared in the vitreous body under the effect of prostaglandin, which was absent under normal conditions.

[Age related changes in the glycosidases of chick embryo eye tissues].

Studies have been made on the activity of glycosidases from eye tissues of developing chick embryos and adult hens. The enzymes of carbohydrates metabolism (hyaluronidase, beta-glycosidase and beta-galactosidase) from the sclera, cornea and ciliary body were examined. It was demonstrated that the distribution of glycosidases in different tissues of the eye is not identical.