Investigation of Biological Activity of Fucoidan and Laminarin as Bioactive Polysaccharides from Irish Brown Macroalgae.

This study aimed to investigate the biological activity of crude and purified laminarin and fucoidan samples extracted from Irish brown macroalgae species and . The antioxidant capacity of the samples was evaluated using the 2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power assays. The anti-inflammatory potential of the samples was analysed using the cyclooxygenases inhibition activity, and the antidiabetic activity was evaluated using a dipeptidyl peptidase-4 inhibitor screening assay.

Elimination of Ethanol for the Production of Fucoidans from Brown Seaweeds: Characterization and Bioactivities.

Fucoidan, a sulphated polysaccharide from brown seaweed composed of several monosaccharides, has been stated to have several bioactive properties such as antioxidant, antiviral, anticancer, antithrombic, anti-inflammatory, and immunomodulatory effects. This paper provides research findings on green extraction methods, structural analysis of fucoidan, and its associated bioactivities. Fucoidans from brown seaweeds, and were extracted using green solvents such as citric acid (CA) followed by MWCO (molecular weight cut-off) filtration to obtain high-purity polysaccharides.

Cellulose nanocrystals/cellulose nanofibrils-combined astaxanthin nanoemulsion for reinforcement of targeted tumor delivery of gastric cancer cells.

Nanoemulsion based nanomaterial (NE) was carried out in the present study to evaluate the efficacy and its antitumor potential of the gastric cancer cells. NE was prepared with astaxanthin/alpha-tocopherol- cellulose nanocrystals/cellulose nanofibrils based nanoemulsions for gastric cancer treatment. The cytotoxic potential was tested against cancer cells and evaluated in terms of its cell proliferation, migration, and cellular uptake by the standard methods.

Ultrasound, microwave and enzyme-assisted multiproduct biorefinery of Ascophyllum nodosum.

This study investigated the multiproduct (fucoidans, β-glucans, proteins, carotenoids, fatty acids, amino acids and polyphenols) valorization of the invasive macroalgae Ascophyllum nodosum within a green biorefinery concept using ultrasound (US), microwave (MW) treatment followed by supercritical CO (SC-CO) with co-solvent and enzymatic extraction. Water and 50% aqueous ethanol were used as green extraction solvents. The extraction methods using 50% ethanol as extraction solvent improved the yields of phenolic compounds and glucan and enhanced in vitro antioxidant activity.

Biological Properties and Health-Promoting Functions of Laminarin: A Comprehensive Review of Preclinical and Clinical Studies.

Marine algal species comprise of a large portion of polysaccharides which have shown multifunctional properties and health benefits for treating and preventing human diseases. Laminarin, or β-glucan, a storage polysaccharide from brown algae, has been reported to have potential pharmacological properties such as antioxidant, anti-tumor, anti-coagulant, anticancer, immunomodulatory, anti-obesity, anti-diabetic, anti-inflammatory, wound healing, and neuroprotective potential. It has been widely investigated as a functional material in biomedical applications as it is biodegradable, biocompatible, and is low toxic substances.

Synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel for drug delivery to gastrointestinal cancer cells.

The present study aims to evaluate the synthesis of nanohydroxyapatite/collagen-loaded fucoidan-based composite hydrogel and characterized its physico-chemical properties for targeted drug delivery to gastrointestinal cancer cells. The nanomaterial is fabricated and characterized as small spherical nanosheets with a high thermal stability by using Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV-vis spectrophotometry (UV-vis), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). in vitro cytotoxicity, BrdU cell proliferation, and scratch assays demonstrate that the nanohydroxyapatite/collagen-loaded fucoidan-based nanomaterial exhibits non-toxicity and increases cell proliferation and migration.

EGFR-conjugated hydrogel accelerates wound healing on ulcer-induced burn wounds by targeting collagen and inflammatory cells using photoimmunomodulatory inhibition.

In the present study, we fabricated an epidermal growth factor receptor (EGFR)-conjugated hydrogel to promote wound healing in cold restraint-induced gastric ulceration on burn wounds targeting collagen and inflammatory cells for the treatment of burns and gastric ulcers. Cytotoxicity and cell proliferation assays demonstrated good biocompatibility of hydrogel as a suitable extracellular matrix for targeted cells and support for regenerative cell growth. These findings were confirmed by staining methods.

Fucoidan-loaded hydrogels facilitates wound healing using photodynamic therapy by in vitro and in vivo evaluation.

The present study aimed at fabrication and evaluation of carboxymethyl cellulose nanofibril-loaded fucoidan/alginate-based gellan gum hydrogel for wound healing with photodynamic approaches. The healing effect of multifunctional hydrogel was characterized and evaluated with in vitro and in vivo methods. Skin tissues were used to perform histological and immunohistochemical analysis to evaluate fibroblasts and collagen content.

Epidermal growth factor receptor conjugated fucoidan/alginates loaded hydrogel for activating EGFR/AKT signaling pathways in colon cancer cells during targeted photodynamic therapy.

In the present study, we developed epidermal growth factor receptor conjugated fucoidan/alginate loaded hydrogels for targeting the delivery of hydrogel through the signaling pathway of the epidermal growth factor receptor (EGFR) to treat colon cancer. We aim to develop a drug delivery system of chlorin e6 encapsulated in hydrogel and tag it with EFGR to target cancer cells with low toxicity and limited side effects by using photodynamic therapy (PDT). The characterization and in vitro studies were conducted to evaluate the efficiency of the EGFR-hydrogel in colon cancer cells.

Cellulose nanocrystals/nanofibrils loaded astaxanthin nanoemulsion for the induction of apoptosis via ROS-dependent mitochondrial dysfunction in cancer cells under photobiomodulation.

The present study investigated effects of low-level laser therapy with cellulose nanocrystals/cellulose nanofibrils loaded in nanoemulsion (NE) against skin cancer cells on apoptosis. The nanoemulsion was fabricated and characterized by the standard methods. The toxicity level by cytotoxicity assays, generation of reactive singlet oxygen (ROS) and antioxidant potential, cell proliferation and migration were confirmed by using standard assays.

Engineering pharmaceutical nanocarriers for photodynamic therapy on wound healing: Review.

In the present scenario, promising efforts have been improved by the researchers towards the development of nanocarriers that can be challenge fundamental tasks in photodynamic therapy (PDT) for wound management. In the past few years, nanotechnology-based therapy has attracted good attention with the advancement of nanotechnology such as PDT, chemotherapy, photothermal therapy. PDT has emerged as a promising clinical treatment method for wound healing.

Nanoengineered chlorin e6 conjugated with hydrogel for photodynamic therapy on cancer.

The aim of the present study is to fabricate hydrogel as a photosensitizer (PS) for photodynamic therapy. Chlorin e6 (Ce6)-fucoidan/alginates@gellam gum (Ce6-Fu/AL@GG)-based hydrogel was fabricated and characterised in terms of morphology and functional groups. MTT assay was used to check toxicity and also performed scratch assay for wound healing property of Ce6-Fu/AL@GGH.

Ultrasound-mediated fucoxanthin rich oil nanoemulsions stabilized by κ-carrageenan: Process optimization, bio-accessibility and cytotoxicity.

This work aims to produce and optimize a κ-carrageenan-based nanoemulsion (NE) to encapsulate seaweed oil, which is rich in fucoxanthin (FX), using ultrasound-assisted emulsification. κ-Carrageenan was produced using subcritical water, and seaweed oil was extracted using supercritical carbon dioxide with sunflower oil as the co-solvent. Response surface methodology (RSM) was used to understand the influence of several process parameters such as ultrasound amplitude, time, temperature, and duty cycle to produce an NE.

A new alternative insight of nanoemulsion conjugated with κ-carrageenan for wound healing study in diabetic mice: In vitro and in vivo evaluation.

Astaxanthin and alpha-tocopherol have attracted great attention because of their properties for the management of wound healing in diabetics. This study aimed to investigate the wound healing study of astaxanthin and alpha-tocopherol with κ-carrageenan nanoemulsion (AS-TP@KCNE) and to perform a histopathological study on streptozotocin (STZ)-induced diabetic mice. AS-TP@KCNE were also evaluated with aim of maintaining an effective and prolonged antidiabetic potential.

In vitro antitumor potential of astaxanthin nanoemulsion against cancer cells via mitochondrial mediated apoptosis.

Astaxanthin and alpha-tocopherol have various biological potential with induction of intracellular ROS production in cytosol, endoplasmic reticulum and mitochondrial site. The present study was performed to prepare nanoemulsion (NEs) formulation of astaxanthin and alpha-tocopherol with sodium caseinate (AS-AT/SC NEs) using spontaneous emulsification and ultrasonication for analyzing intracellular ROS production in apoptosis. NEs was characterized with standard analysis, which revealed a high stability of normal pH with a small size and unique zeta potential of spherical structure droplets with no toxicity and faster cell migration.

Astaxanthin-alpha tocopherol nanoemulsion formulation by emulsification methods: Investigation on anticancer, wound healing, and antibacterial effects.

Emulsion-based delivery systems have been fabricated and developed to increase the bioavailability of astaxanthin and alpha-tocopherol as active compounds for various biomedical applications. Astaxanthin-alpha tocopherol nanoemulsion (ATNE) is well known for its potential 6.-6.

Fabrication of multifunctional chitosan-based nanocomposite film with rapid healing and antibacterial effect for wound management.

A nanocomposite film, chitosan (CS)-polyvinylpyrrolidone (PVP)-bentonite (BN) was fabricated to enhance wound healing processes as a new nanoplatform for wound dressing. Both physical properties and antibacterial activity of the proposed film were examined to validate its applicability and inhibitory effect for wound management. In vitro cytotoxicity was evaluated by using MTT assay on L929 and NIH3T3 cells to identify the toxicity level of the film.