Development of Two-Tube Loop-Mediated Isothermal Amplification Assay for Differential Diagnosis of and and Its Comparison with Loopamp™ Malaria.

To strengthen malaria surveillance, field-appropriate diagnostics requiring limited technical resources are of critical significance. Loop-mediated isothermal amplification (LAMP) based malaria diagnostic assays are potential point-of-care tests with high sensitivity and specificity and have been used in low-resource settings. -specific consensus repeat sequence (CRS)-based and -specific 18S rRNA primers were designed, and a two-tube LAMP assay was developed.