[Analysis of the mutagenic activity of aqueous and buffer extracts from oil-polluted soil samples].

The Ames test on Salmonella typhimurium TA 100 and TA 98 strains were used to study the mutagenic activity of aqueous (pH 7.4) and buffer (pH 4.5) extracts from soil samples containing different oil levels (from 16 to 3526 mg per kg soil), which had been taken from different areas of a town, and buffer extracts from model soil samples added by crude West-Siberian oil (160-15625 mg/kg).

[Hygienic evaluation of the effect of petroleum hydrocarbons on the soils of megapolises].

The paper gives the results of studying the quantity of petroleum hydrocarbons in the Moscow soil, as well as the latter's hygienic evaluation by the changes in acidity (pH) and redox potential and by the sanitary state (the levels of Escherichia coli and viable helminthic eggs). The study has ascertained that the petroleum-polluted soils show changes in medium pH and redox conditions that determine the biological activity of the substances and the trend in their disintegration processes and revealed a relationship of thy sanitary state of Moscow soils to their level of petroleum hydrocarbons.

[Oil pollution of a number of Moscow residential and recreational areas].

The soils of residential areas were studied in different Moscow districts differing in the level of a man-caused load. Oil pollution was estimated by the soil content of oil hydrocarbons. A correlation was established between the levels of hydrocarbons and the sanitary ill-being of the city's soils.

[The environmental-and-hygienic risk of industrial waste and consumption].

How to safely handle production and consumption residues is a relatively recent problem. According to the data of the United Nations Organization, 25 to 33% of the diseases notified in the world are directly related to the poor human environment. In Russia and foreign countries, there has been no list of chemicals so far.

[Topical problems of society's waste handling].

The fact that the volume of waste steadily grows, makes its handling one of the most topical scientific, technical, and socioeconomic problems in the world. The article covers important ecological and hygienic problems of waste handling and the effects of waste of human health.

[Parameters of platelet serotonin system in patients with schizophrenia treated with olanzapine: a search for the serotonergic predictors of therapeutic efficacy].

Fifty-nine patients, 21 women and 38 men, with ICD-10 diagnosis of schizophrenia (F20.0), attack-like type, were treated with olanzapine during 28 weeks (8-weeks of acute and 20-weeks of maintenance therapy). Evaluation of clinical symptoms measured by the Positive and Negative Syndromes scale (PANSS) revealed that female patients responded better to therapy as compared to male ones, with PANSS total, PANSS negative and PANSS general psychopathological scores being significantly reduced (p < 0.

[Barrier role of water-purifying construction of water pipe in relation to opportunistic microorganisms].

The investigations have indicated that in the flood period when the water-purifying means of a water pipe bears the maximum load, the existing water-preparing system cannot fully purify and disinfect drinking water from some opportunistic microbes (Klebsiella, Acinetobacter). The latter were recorded in the pipe water having the MAC of coliform organisms established by the GOST.

Autoimmune component in individuals during immune response to inactivated combined vaccine against Q fever.

Serum samples from 20 individuals immunized with inactivated combined vaccine (ICV) against Q fever and 10 individuals that received placebo were investigated on days 14, 21, 28 and 60 after immunization by isotope specific enzyme-linked immunosorbent assay (ELISA) for the presence of antibodies directed to human IgA, IgM and IgG, and their fragments (F(ab')2, Fab, Fc). None of the subjects that received placebo exhibited significant increase of reactivity with any of the used antigens. By contrast, the sera of immunized individuals tended to show increased autoantibody activity with diverse antigens.

[The morphological changes in the body of guinea pigs in response to the administration of different Coxiella burnetii antigens].

In this work the evaluation of macro- and micro-changes in the tissues of guinea pigs to the injection of inactivated antigens of C. burnetii is presented. The injection of these antigens in a dose of 50 micrograms induced only an insignificant reaction at the site of injection; in the brain of the animals, as well as in all examined internal organs, no visible pathological changes were present.

[Characteristics of DNA restriction fragment length polymorphisms of C. burnetti strains, isolated from areas of Russia].

The structural heterogeneity in Coxiella burnetii chromosomal DNA isolated in the European part of Russia from people, agricultural animals, and ticks has been studied. It is compared with the one of the European strains Henzerling and M44, the only genetically characterized strains up to date. The digestion of the total DNA by the restriction endonucleases BamHI, PstI, XhoI resulted in obtaining two types of restriction patterns.

Biological properties of chloroform/methanol extracts of Coxiella Burnetii.

Biological properties of antigens of whole cells of C. burnetii in phase I (WCI), their residue (CMR) and extract (CME) obtained by extraction of C. burnetti with chloroform/methanol mixture were subjected to a comparative study.

Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice.

C3HA mice were inoculated intraperitoneally (i.p.) with viable Coxiella burnetii (C.

[The host reaction to the administration of different components of Coxiella burnetii].

After the intraperitoneal injection of corpuscles of C. burnetii antigen (Ag), phospholipid (PL), and sediment obtained after the extraction of PL from Ag with chloroform-methanol (CM) slight leukocytic reaction developed in the peritoneum on day 1, and on day 2 it could be observed in the liver and in the spleen. Ag induced the most pronounced morphological changes.

[An immunoenzyme method for demonstrating Coxiella burnetii antigens].

Materials on the development of an enzyme immunoassay (EIA) system for the detection of the antigens of C. burnetii, the causative agent of Q rickettsiosis, are presented. The system is highly specific and effective with respect to both corpuscular antigens of phases 1 and 2 and soluble antigen (lipopolysaccharide).

[Optimization of culinary recipes according to amino acid composition].

A computation method is suggested, enabling one to optimize the amino acid composition of protein mixtures proceeding from the real content of amino acids and protein assimilation ratios. The method is based on the adequate use of essential amino acids and an amino acid that limits the biological value of proteins. The difference between the real content of essential amino acids and the portions utilized constitutes a non-utilized part of protein.

[Interaction of live and killed Coxiella burnetii with model animal cells at the ultrastructural level].

Materials are submitted on electron-microscopic studying of alive and inactivated Coxiella in their interaction with murine cells during 28 days observations. The most variability of Coxiella morphologic types is shown to be found in spleen. The structure of endospora type is described.

[Immunogenic and immunomodulating properties of phospholipids from Coxiella burnetii].

It is proved that the challenging of the extract isolated from Coxiella (as a result of treatment with the mixture of chloroform and methanol) to animals doesn't provoke the appearance of antibodies to these microorganisms. It also doesn't cause the sensibilization of lymphocytes to Coxiella, but the extract can interact specifically with a serum containing antibodies to Coxiella. It permits to characterise the tested extract of Coxiella as a hapten with properties of adjuvant because it stimulates antibody-appearance.