Derivatization-Based Densitometric Approach Along With Greenness Assessment for Simultaneous Evaluation of Duloxetine Hydrochloride and Pregabalin: An Application to Pharmaceutical Analysis.

The fixed-dose combination of pregabalin and duloxetine hydrochloride is mainly used in the treatment of neuropathic pain. Sensitive determination of drugs like pregabalin is challenging by chromatographic methods using UV detection due to their nonchromophoric nature. This study presents the development of a validated high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination pregabalin and duloxetine hydrochloride in formulation.