Standardization of polymerase chain reaction for detection of fluconazole resistance targeting Y132F mutation in gene in .

Background And Purpose: is the third most commonly isolated species from candidemia patients admitted to Indian intensive care units. Outbreak of infection and emergence of fluconazole resistance associated with this particular species has been increasingly documented since 2018. Worldwide data has documented that Y132F substitution in the gene is the predominant fluconazole resistance mechanism among .

Evaluating soil quality and carbon storage in Western Ghats Forests, Karnataka, India, for sustainable forest management.

Monitoring soil quality index (SQI) and soil organic carbon (SOC) stock status of the Western Ghats (WG) forests in India is crucial for providing vital ecosystem services alongside sustainable forest management practices. However, comprehensive profile data on SQI and SOC stock across different forest types under WG forests are limited. The study evaluated SQI and SOC stock under three forest types, i.

Theoretical, structural, and electronic analyses of pyridin-based dyes for dye-sensitized solar cells applications.

Context: The new series of donor-π-acceptor dyes have been designed using pyridine derivatives as a donor group and thienothiophene as a π-spacer group, which were linked via 10 acceptor groups. The highest occupied molecular orbital energies range from - 6.177 to - 5.

Production of Virulence Factors and Antifungal Susceptibility Pattern of Isolates from Clinical Samples in a Tertiary Care Center.

 This study was aimed to investigate the association between virulence factors and antifungal susceptibility pattern among species.  This study was carried out in the Department of Microbiology, from May 2018 to June 2019. A total of 52 isolates obtained from various clinical samples were speciated based on microscopic identification by lacto phenol cotton blue (LPCB) mount and slide culture technique.

Draft Genome Sequences of the Kocuria subflava Type Strain KCTC 39547 and sp. Strain JC486, a Newly Isolated Strain from a Wild Ass Sanctuary in Gujarat, India.

Here, we report a 2.86-Mbp genome sequence of sp. strain JC486, which was isolated from a salt marsh, and a 3.

Prognostication of the neurological outcome of tethered cord based on intraoperative neuromonitoring findings: how close can we get?

Purpose: To evaluate the correlation of intraoperative neuromonitoring (IONM) data in surgery for tethered cord syndrome (TCS) in children to the neurological outcome at 1-year follow-up.

Methods: 208 consecutive patients operated on for TCS, between January 2011 to February 2020, under electrophysiological monitoring in the Division of Paediatric Neurosurgery, AIMS, Kochi, India, were included. Their preoperative neurological, urological and orthopaedic status were compared with the postoperative status at 1 year follow-up.

Green synthesis of gold nanoparticles using extracellular metabolites of fish gut microbes and their antimicrobial properties.

In the present study, we synthesis nanoparticles using biosynthesis methods because of the eco-friendly approach. Gold nanoparticles were synthesized using extracellular metabolites of marine bacteria (Rastrelliger kanagurta, Selachimorpha sp., and Panna microdon).

Evaluation of a labdane diterpene forskolin isolated from (Lam.) Gandhi (Cucurbitaceae) revealed promising antidiabetic and antihyperlipidemic pharmacological properties.

(Lam.) Gandhi (Family Cucurbitaceae) is one of the important plant species used by the Irula tribes of Walayar valley of southern Western Ghats, India for the management of diabetes. To confirm the antidiabetic property of , the present study was addressed using crude methanolic leaf extract of (MeOHSa) and its isolated compound, Forskolin against streptozotocin (STZ) induced diabetic rats.

The potential of antioxidant activity of methanolic extract of (Goetgh.) Colebr (Menispermaceae).

To explore the possible bioactive compounds and to study the antioxidant capacity of (Goetgh.) Colebr (Menispermaceae) the qualitative and quantitative phytochemical screening for various secondary metabolites were evaluated. Using the GC-MS analysis, a total number of 30 phytochemical compounds were predicted with their retention time, molecular weight, molecular formula, peak area, structure and activities.

Pharmacological credence of the folklore use of in the management of jaundice.

The information on the hepatoprotective effect of Roxb. (Family Leguminosae) used in the folkloric medical practice in Malabar coast and Walayar valley of southern India for the treatment of liver related disorders is completely unknown. Hence, the efficacy of the aqueous methanolic extract of stem bark of (AqMeOH-) was evaluated for liver function serum biochemical markers along with the antioxidant markers in liver tissues of Wistar albino rats.

Serodiagnosis of Scrub Typhus at a Tertiary Care Hospital from Southern India.

Introduction: Scrub typhus, a zoonotic disease is one of the most covert emerging and re-emerging Rickettsial infections. There is an upsurge in the incidence of the disease worldwide with ever-changing habitat. Clinical diagnosis of scrub typhus is challenging as the signs and symptoms of scrub typhus are similar to other febrile illnesses.

TLC and HPTLC Fingerprints of Various Secondary Metabolites in the Stem of the Traditional Medicinal Climber, Solena amplexicaulis.

Aim of this study was to develop a TLC and a HPTLC fingerprint profiles for various secondary metabolites of methanol extracts of the stem of the traditional medicinal climber, Solena amplexicaulis. These studies were carried out as per the methods of Harborne and Wagner et al. The profiles of various individual secondary metabolites were made and developed for authentication.

Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities.

Objective: To analyse qualitative and quantitative phytochemical and evaluate in vitro antioxidant properties of various alcoholic and aqueous extracts of leaf and root parts of Hypochaeris radicata.

Methods: Preliminary phytochemical analysis for alkaloids, cardiac glycosides, flavonoids, glycosides, phenols, resins, saponins, steroids, tannins, terpenoids and triterpenoids and quantitative phytochemical analysis for alkaloids, total phenolics, total flavonoids, tannins, saponins and ascorbic acid were made by following standard procedures. In vitro antioxidant properties were evaluated by assessing DPPH(•), NO(•) and ABTS(•+), radical scavenging abilities and assaying the reducing power, β-carotene and antihemolytic activities by adapting standard methods.

Hydrolysis of acid and alkali presoaked lignocellulosic biomass exposed to electron beam irradiation.

In this study, synergetic effect of mild acid and alkali with electron beam irradiation (EBI) on the enzymatic hydrolysis of a selected grass biomass was assessed. Biomass samples prepared by soaking with 1% H2SO4, or 1% NaOH, were exposed to 75 and 150 kGy of EBI. Water presoaked biomass was used as control.

Enzymatic hydrolysis and characterization of lignocellulosic biomass exposed to electron beam irradiation.

Pretreatment of lignocellulosic biomass has been taken up as a global challenge as it comprises a large renewable source of fermentable sugars. In this study, effect of electron beam irradiation (EBI) on a hybrid grass variety investigated as a biomass pretreatment method. Dry biomass samples after characterization were exposed to EBI doses of 0, 75, 150 and 250 kGy.