The issue of vaccine refusal: study of risky behaviour.

Background: The aim of this study is to identify the influence of factors that determine the refusal of influenza vaccine among three subjects groups.

Methods: A survey was conducted amongst three high‑risk groups in years 2018-2019 (Moscow, Russia). The survey involved 1,620 parents and pregnant women (group 1), 324 doctors (group 2) and 433 students (group 3).

[Effect of Prostanorm on the persistent potential of microorganisms isolated from patients with chronic bacterial prostatitis].

Aim: To determine the effect of the Prostanorm on the persistent potential of microorganisms isolated from the prostatic secretion of patients with chronic bacterial prostatitis.

Materials And Methods: In vitro experiments were carried out on uropathogens, isolated from prostate secretions in patients with chronic bacterial prostatitis, including Escherichia coli, Staphylococcus aureus, S. haemolyticus, S.

[Effect of Fitofron on the persistence factors of opportunistic pathogens in urine isolated from patients with urinary tract infections].

Aim: To perform an experimental evaluation of the effect of Phytofron, used for the treatment of urinary tract infections, on the ability of opportunistic pathogens to inactivate innate immunity factors (lysozyme, pro- and anti-inflammatory cytokines) and form biofilms.

Materials And Methods: In vitro experiments were carried out on clinical isolates from urine of patients with pyelonephritis and cystitis: Escherichia coli, Staphylococcus aureus, S. haemolyticus, S.

Role of gut microbiota in the modulation of the health effects of advanced glycation end‑products (Review).

The aim of the present review was to summarize the potential interactive effects between the gut microbiota and advanced glycation end‑product (AGE) accumulation and toxicity in the host, and to reveal potential the mediatory effects of the gut microbiota on AGE‑related health effects. The existing data demonstrate that dietary AGEs can have a significant impact on the richness and diversity of the gut microbiota, although the particular effect is dependent on the type of species, as well as the exposure dose. In addition, the gut microbiota may metabolize dietary AGEs.

[Antibiotic resistance of strains of gram-positive cocci isolated from prostate secretion in men with chronic bacterial prostatitis].

Objective: to characterize the antibiotic resistance of gram-positive cocci strains isolated from the prostate secretion in men with chronic bacterial prostatitis at the level of phenotype and genotype.

Materials And Methods: Bacteria were isolated from the prostate secretion of men of reproductive age (20-45 years) with chronic bacterial prostatitis by conventional bacteriological method. The type of microorganisms was determined by MALDI-TOF mass spectrometry.

The modern Russian pharmaceutical market: consumer attitudes towards distance retailing of medicines.

Background: In Russia, remote retail trade of over-the-counter (OTC) medicines was legalised. According to statistics as of April 2020, consumer demand in the categories of "online pharmacies" increased by 803%.

Methods: The study was conducted in two stages by cross-sectional method using a structured questionnaire in the central region of Russia: 1st stage - July-August 2020; 2nd stage - February-March 2021.

Effect of combined glucocorticoid therapy on bronchial asthma dynamics.

This work is aimed to compare the effectiveness of parenteral and inhalation bronchial asthma treatment in combination with glucocorticosteroids and bronchodilators. The study was conducted in 2020 in Botkin City Clinical Hospital (Moscow, the Russian Federation). Case histories of 106 patients diagnosed with bronchial asthma exacerbation of moderate severity were analyzed.

Specific features of rational pharmacotherapy in elderly patients.

Objective: It is well-known that finding an optimum medication at the correct dose for elderly patients is challenging for the practitioner. This study aimed to examine the main trends in prescribing medications for elderly patients and their compliance with the principles of rational pharmacotherapy, and to establish the main factors affecting adherence to treatment in these patients.

Methods: 956 records of outpatients over 60 years of age were examined.

Genome Analysis of the Probiotic Strain Enterococcus faecium Ef79OSAU.

We report here the draft genome sequence of Enterococcus faecium strain Ef79OSAU, which was isolated from swine feces. The characteristics of strain Ef79OSAU reveal the absence of pathogenicity factors, a wide range of antimicrobial activity , and antilisteriosis activity . Analysis of the E.

[Characteristics of the pathogenic potential of Escherichia coli isolated from patients with calculous pyelonephritis].

Objective: Comparative phenotypic and genetic assessment of the pathogenic potential of E. coli strains isolated from patients with calculous pyelonephritis.

Materials And Methods: 78 strains of E.

Medical and Social Factors of Pediculosis.

Pediculosis is a global problem in public health. An important factor in the efficient eradication of lice is ensuring adequate recognition and treatment of the disease by the population. In the present study, awareness of the population about the physiological properties of head lice, the ways of infestation with head lice, and the methods of treatment and prevention were studied.

Genomic Sequence of the Strain Enterococcus faecium ICIS 18.

We report here the draft genome sequence of strain ICIS 18, which was isolated from human feces. Analysis of the ICIS 18 genome revealed genes encoding resistance to metals, fluoroquinolones, and beta-lactam antibiotics.

[Biological properties of microorganisms isolated from the urine of patients with urolithiasis].

Aim: To define persistent properties and antibiotic resistance of microorganisms isolated from the urine of adult patient undergoing surgery for urolithiasis.

Materials And Methods: Urine specimens were obtained from the renal pelvis and urinary bladder during percutaneous nephrolithotripsy. Microorganisms that were isolated from the urine were examined for their persistent properties (anti-lysozyme activity, the ability of biofilm formation) and antibiotic resistance using photometric and bacteriological methods.

Genome Sequence of Enterococcus faecium Strain ICIS 96 Demonstrating Intermicrobial Antagonism Associated with Bacteriocin Production.

We report here the complete genome sequence of strain ICIS 96, which was isolated from the feces of a horse. Bacteriological characterization of strain ICIS 96 revealed the absence of pathogenicity factors, while its spectrum of antagonistic activity was found to be broad, having activities associated with both Gram-positive and Gram-negative bacteria. Analysis of the ICIS 96 genome revealed five genes associated with antimicrobial activity (enterocin [ent] A, ent B, lactobin A/cerein 7b, and ent L50 A/B).

Antimicrobial activity of the indolicidin-derived novel synthetic peptide In-58.

Natural peptides with antimicrobial activity are extremely diverse, and peptide synthesis technologies make it possible to significantly improve their properties for specific tasks. Here, we investigate the biological properties of the natural peptide indolicidin and the indolicidin-derived novel synthetic peptide In-58. In-58 was generated by replacing all tryptophan residues on phenylalanine in D-configuration; the α-amino group in the main chain also was modified by unsaturated fatty acid.

[Pharmaceutical care for patients of the third age in pharmacy organizations as a significant aspect of social gerontology].

There are questions in the article that study mutual relations of patients of elderly and senile age with pharmaceutical experts. It was revealed that pharmacy organizations are an important element in the life of patients of the third age and noted the high role of the pharmaceutical specialist in their social environment. The article shows the need to focus on the psychosomatic features of elderly patients with proper pharmaceutical counseling and professional communication.

[Structure of microorganism species cultured from urine of urolithiasis patients].

Aim: To investigate the incidence of microorganisms of different taxonomic groups and their associations in the pelvic and bladder urine of adult urolithiasis patients.

Materials And Methods: A bacteriological method and metagenomic sequencing were used to investigate the bacterial spectrum of microflora cultured from pelvic and bladder urine sampled during surgical interventions in urolithiasis patients.

Results: The both microbiotas had approximately the same spectrum, but in 26.

Novel haemoglobin-derived antimicrobial peptides from chicken (Gallus gallus) blood: purification, structural aspects and biological activity.

Aim: To purify and characterize antimicrobial peptides derived from the acid extract of Gallus gallus blood cells.

Methods And Results: Two polypeptides (i.e.

[BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES FROM CHICKENS THROMBOCYTES].

Aim: Isolation and study of biological activity of antimicrobial peptides from chickens thrombocytes.

Materials And Methods: Peptides from chickens thrombocytes, obtained by reverse-phase high-performance liquid chromatography method with stepped and linear gradients of concentration increase of the organic solvent were used in the study. Their antimicrobial activity was determined by microtitration method in broth; mechanism of biological effect--by using fluorescent spectroscopy method with DNA-tropic dyes.

[Antibiotic Resistance of Enterococci Isolated from Healthy Humans and Patients with Various Pathologies.].

High resistance of enterococci to the currently used antibacterials, such as tetracycline, ciprofloxacin and ampicillin was observed. Streptomycin, gentamicin and vancomycin showed the highest activity against the clinical isolates of Enterococcus spp. Streptomycin and gentamicin showed the highest activity against the intestinal enterococci.

[PHENOTYPICAL CHARACTERISTICS AND GENETICAL DETERMINANTS OF PATHOGENICITY OF STAPHYLOCOCCUS AUREUS, ISOLATED FROM BACTERIAL CARRIERS, RESIDING ON THE TERRITORIES WITH VARIOUS LEVELS OF ANTHROPOGENIC POLLUTION OF AIR ENVIRONMENT].

Aim: Comparative phenotypical and genetical evaluation of pathogenic potential of Staphylococcus aureus strains, isolated from resident bacterial carriers, residing on the territories with anthropogenic pollution of air environment of varying intensity.

Materials And Methods: S. aureus, isolated 3 times from mucous membrane of the anterior of nose from 210 children, were the object of the study.

[BIOLOGICAL PROPERTIES OF ENTEROCOCCI OF VARIOUS ORIGINS].

Aim: Study the prevalence and intensity of anti-hemoglobin and anti-lactoferrin activity in enterococci as representatives of symbiotic intestine microflora and causative agents of infection-inflammatory diseases in children.

Materials And Methods: 95 strains of enterococci were used in the study, that were isolated from intestines of children during examination for dysbiosis, and 34 clinical isolates. Strain identification was carried out by using multiplex PCR.

Morphofunctional reaction of bacteria treated with antimicrobial peptides derived from farm animal platelets.

Classical microbiological approach and atomic force microscopy were used to evaluate the mechanisms of biological activity of antimicrobial peptides (AMPs) derived from platelets of farm animals. It is established that AMPs inhibit both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) microorganisms. Differences revealed in the biological activity of AMP preparations obtained from the organisms of various species can be reduced to quantitative differences.

[Cycloferon biological activity characteristics].

Aim: Study the effect of cycloferon in experimental and clinical conditions on persistence properties of aurococci as well as features of their morpho-functional reaction by atomic force microscopy.

Materials And Methods: The study was carried out in 12 Staphylococcus aureus clones isolated from mucous membrane of nose anterior part of a resident carrier. The effect of cycloferon in vivo was evaluated in 26 resident staphylococci carriers under the control of anti-carnosine activity of staphylococci.

[Characteristic of microflora isolated during mechanical lung ventilation].

Aim: Study species composition and biological properties of microorganisms isolated from lower respiratory tract of 34 patients of surgical departments during mechanical lung ventilation.

Materials And Methods: 59 strains of microorganisms were isolated and identified by established methods from tracheobronchial secretion during intubation and extubation. Hemolytic and anti-lysozyme activity was determined in isolates by methods described previously (O.