The effects of cholestyramine, colestipol, and ADR-132 on the rat prostate and dunning R-3327 adenocarcinoma.

The effects of three compounds known to have hypocholesterolemic activity in several species were investigated on the rat prostate and the hormone-dependent R-3327 rat prostatic adenocarcinoma. Cholestyramine, colestipol, and ADR-132 are bile acid-sequestering anion exchange resins which were fed to separate groups of adult male Copenhagen X Fischer (F1) hybrid rats in doses of 0.25%, 1.

LNCaP model of human prostatic carcinoma.

The LNCaP cell line was established from a metastatic lesion of human prostatic adenocarcinoma. The LNCaP cells grow readily in vitro (up to 8 x 10(5) cells/sq cm; doubling time, 60 hr), form clones in semisolid media, are highly resistant to human fibroblast interferon, and show an aneuploid (modal number, 76 to 91) human male karyotype with several marker chromosomes. The malignant properties of LNCaP cells are maintained.

Effect of tamoxifen citrate on a murine renal cell adenocarcinoma.

High-dose tamoxifen citrate (80 mg/kg), or estradiol (100 micrograms/kg) was administered to intact or castrated Balb/C male and female mice with implanted murine renal cell adenocarcinoma. 100% tumor growth occurred, and tumor weights in tamoxifen treated (mean 3.69 g) and untreated (mean 3.

Serum prostatic antigen measurement in localized prostatic cancer: correlation with clinical course.

Serial serum prostatic antigen measurements were performed on patients treated for localized prostatic carcinoma. There was good correlation of mean serum prostatic antigen levels and the surgical stage of the disease. Among patients treated by radical prostatectomy there was good correlation of an elevated serum prostatic antigen and the development of metastatic disease.

Effects of estramustine and megestrol on ultrastructure of rat ventral prostate.

The effects of estramustine phosphate on the ultrastructure of the rat ventral prostate closely resemble postcastration events, i.e., a decrease in cell size and secretory activities.

Estrogen receptors and clinical correlations with human prostatic disease.

Measurement of estrogen binding in human prostate using high-pressure liquid chromatography (HPLC) revealed the presence of cytosolic estrogen receptors (ER) both in benign prostatic hyperplasia (BPH) and adenocarcinoma. Receptor concentrations correlated with several histopathologic features in the specimens analyzed. Estrogen receptor levels generally were higher in BPH than in cancer specimens although there was a subgroup of patients with poorly differentiated carcinoma with levels higher than those of BPH, HPLC can be used for measuring ER in 50 microliters of cytosol, and thus needle biopsy specimens will be analyzed routinely for ER with this micromethod.

Correlation of histochemical and biochemical analyses of androgen binding in prostatic cancer: relation to therapeutic response.

A histochemical technique for the detection of androgen binding in prostatic cancer was performed on specimens from 108 patients and compared with a biochemical method in a double blind study of 77. Statistical analyses showed a significant agreement between the two assay systems for the qualitative and quantitative presence or absence of specific androgen binding, as well as for the subcellular localization of binding in nucleus and/or cytoplasm. Although the number of cases studied was too small for statistical analysis, there appeared to be good correlation between histochemical androgen binding results and clinical response, or lack of response to hormonal manipulation in 20 patients with State C and Stage D carcinoma.

Effects of DES on the morphology of the lobes of the baboon prostate.

Ultrastructural examination of the baboon prostate has revealed differences between the caudal and cranial lobes. The cranial epithelium is characterized by electron dense, tall columnar cells which contain numerous secretory vacuoles of homogenous appearance; the caudal epithelium is comparatively shorter in height and lighter in electron opacity. The caudal cells also contain numerous secretory granules which vary in their characteristics.

Estramustine binding in rat, baboon and human prostate measured by high pressure liquid chromatography.

High pressure liquid chromatography (HPLC) was used to determine 3H-estramustine (estradiol-17 beta 3N-bis-[2-chlorethyl] carbamate), 3H-17 beta-hydroxy-5 alpha-androstan-3-one (3H-dihydrotestosterone or 3H-DHT), 3H-estradiol-17 beta (3H-E2) and 3H-3 beta-hydroxy-5-pregnen-20-one (3H-pregnenolone) binding in 50(2) microliter of cytosol utilizing a column which separates proteins in the molecular weight range of 2,000 to 70,000 daltons. The rat prostate contains a protein in considerable concentration and with the highest affinity for estramustine (375,000 dpm 3H-estramustine per mg. cytosol protein) among the substances tested.

Use of silastic implants to raise plasma androgen levels in the immature baboon (Papio cynocephalus).

Two immature Papio cynocephalus baboons were implanted with silastic tubing containing testosterone (T) to obtain prolonged elevation of plasma androgen levels. Plasma radioimmunoassay indicated a thousandfold increase of T levels over baseline for three weeks. Two weeks after implant removal, plasma androgen levels were at, or below, the pre-implantation levels.

Effects of diethylstilbestrol and estramustine phosphate on serum sex hormone binding globulin and testosterone levels in prostate cancer patients.

Serum testosterone-estradiol binding globulin and total testosterone were measured in 2 groups of male controls (less than 50 and more than 65 years old) and in 7 groups of prostatic cancer patients treated with various endocrine manipulation procedures, including orchiectomy, and estramustine phosphate and diethylstibestrol therapy. There were 133 individuals studied. Total serum testosterone levels were significantly higher in the younger versus the older control group and testosterone-estradiol binding globulin levels were significantly higher in the older men.

An assessment of bone scans for monitoring osseous metastases in patients being treated for prostate carcinoma.

The National Prostatic Cancer Project held a workshop in March 1980 to examine procedures used to evaluate bone metastases. Variations in techniques used by each of 9 treatment centers, as well as the National Cancer Institute, were compared. One center in particular had developed procedures for quantitating bone scans in addition to the customary qualitative evaluation.

Pesticides and PCB residues in the Black Creek Watershed, Allen County, Indiana--1977-78.

The aquatic environment of a small agricultural drainage in northeastern Indiana was surveyed during 1977-78 for pesticide and PCB contamination. A total of 45 water, sediment, and fish samples from Black Creek watershed, Allen County, Indiana were analyzed for seven pesticides and PCBs. Low levels of dieldrin (mean 0.

Steroid hormone receptors in the prostate.

Specific receptors for dihydrotestosterone and estradiol-17-beta have been identified in cytosols of the human and baboon prostate. Binding of radioactive estradiol-17-beta to the 0.4 M potassium chloride extractable component of human prostate nuclei also was demonstrated.

Androgen binding in the baboon prostate.

Androgen receptors were identified and partly characterized in cytosols of the caudal and cranial prostatic lobes of a 24-hr castrate baboon. Binding of cyproterone acetate (CA) to testosterone-binding globulin (TeBG) in baboon serum was negligible and therefore was an appropriate unlabeled competitor for distinguishing high-affinity binding of tritiated dihydrotestosterone (3H-DHT) to serum contaminants and receptors in cytosol preparations when multiple-point saturation analyses and removal of free steroid by charcoal adsorption were used. Specificity of androgen binding was demonstrated by the inability of diethylstilbestrol, a synthetic estrogen known to have low binding affinity for TeBG, to displace 3H-DHT from the receptor protein.

Water resources and the land-water interface.

Development and implementation of local and regional plans to control nonpoint sources of pollution from agricultural land are major mandates of section 208 of Public Law 92-500. Many planners tend to equate erosion control as measured by the universal soil loss equation with improvements in water quality. Others implement channel management practices which degrade rather than improve water quality and thereby decrease the effectiveness of other efforts to control nonpoint sources.