Sequential cultivation method for β-fructofuranosidase production from URM4634, evaluation of their biochemical and kinetic/thermodynamic characteristics, and application on sucrose hydrolysis.

The present study focused on evaluating the sequential fermentation (SF) method for FFase production from URM4634 using soybean bran as substrate. The SF was performed using soybean bran as substrate at 72 h and 30 °C and the maximum hydrolytic activity (44.00 U mL), corresponding to an increase of 2.