Publications by authors named "Karolina Wawro"
The members of the ZC3H12/MCPIP/Regnase family of RNases have emerged as important regulators of inflammation. In contrast to Regnase-1, -2 and -4, a thorough characterization of Regnase-3 (Reg-3) has not yet been explored. Here we demonstrate that Reg-3 differs from other family members in terms of NYN/PIN domain features, cellular localization pattern and substrate specificity.
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- - DNA gyrase is a bacterial enzyme that is targeted by natural toxins and synthetic drugs, like fluoroquinolones, but resistance can occur through proteins known as pentapeptide repeat proteins (PRPs).
- - The study reveals that a specific PRP, QnrB1, protects against fluoroquinolones by interacting with the GyrB protein and enhancing its ATP hydrolysis activity.
- - Researchers identified the binding site of QnrB1 on GyrB and suggest that its binding helps release fluoroquinolones from their action site, thereby contributing to antibiotic resistance.
ZC3H12B is the most enigmatic member of the ZC3H12 protein family. The founding member of this family, Regnase-1/MCPIP1/ZC3H12A, is a well-known modulator of inflammation and is involved in the degradation of inflammatory mRNAs. In this study, for the first time, we characterized the properties of the ZC3H12B protein.
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- ADAM17 is a metalloproteinase that helps release various proteins from cell surfaces and is often overexpressed in tumors and during inflammation.
- Research investigates how ADAM17's expression is regulated by transcription factors NF-κB and Elk-1, specifically when exposed to pro-inflammatory stimuli.
- Key findings show that NF-κB plays a crucial role in ADAM17 expression, while the ERK1/2 pathway and Elk-1 also significantly influence its regulation, including identifying specific Elk-1 binding sites in the ADAM17 promoter.
Both ADAM17, the secretase responsible for the shedding of ectodomains of numerous membrane proteins including TNF and its receptors, as well as nitric oxide synthesized by inducible nitric oxide synthase play regulatory roles in inflammation and tumor progression. We analyzed the effect of endogenous and exogenous nitric oxide on the expression and activity of ADAM17 in murine endothelial cells and a monocyte/macrophage cell line. We found that endogenous nitric oxide influenced neither ADAM17 mRNA level nor the shedding of two ADAM17 substrates, TNF and TNFR1.
View Article and Find Full Text PDFPlasmacytoid dendritic cells (pDC), so named because of histological similarities to antibody-producing plasma cells, comprise a unique dendritic cell population. Despite their low numbers in blood they play a key role in anti-viral immunity, mainly through secreting massive amounts of type 1 interferon following an engagement of their Toll-like receptors, such as TLR7 and TLR9, by viral nucleic acids. In a mature state, pDC display a propensity to present antigens to naive lymphocytes.
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