Effect of focal cerebral ischaemia on modulatory neurotransmitter receptors in the rat brain: an autoradiographic study.

Neurotransmission is strongly affected after ischaemic insult. It is postulated that modulatory neurotransmitter systems and their receptors play a role in experience-dependent and restoration plasticity. In this study, muscarinic cholinergic, serotonergic 5-HT(2A/2C), dopaminergic D(1) and noradrenergic beta(1) receptors were examined after focal cerebral ischaemia in different brain regions, using quantitative in vitro autoradiography.

Spatiotemporal dynamics of astroglial and microglial responses after photothrombotic stroke in the rat brain.

The effects of photothrombotic stroke in primary somatosensory cortex on astroglial and microglial activation in various regions of lesioned brain were examined at different time points, using immunohistochemistry and lectin binding. The increase in GFAP expression was observed exclusively in the ipsilateral hemisphere, both in the perilesional area and cortical regions distant from the infarct. This remote increase was detectable up to sixty days after the infarct.

D1 dopamine receptors distribution following photothrombotic stroke in rat cerebral cortex.

The effect of focal photothrombotic stroke on the distribution of D1 dopamine receptor (D1R) sites was examined in different cortical areas of rat brain with quantitative receptor autoradiography using [3H]SCH23390 as a ligand. Unilateral cortical stroke was located in the primary somatosensory cortex. After different survival times (1, 7 and 28 days) D1R binding levels were determined in the lesion core, penumbra, frontoparietal motor (FrPaM) and somatosensory (FrPaSS) areas as well as in homotopic regions in the contralateral hemisphere.