Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm.

This study investigated the effect of glycerol added in different phases of sperm equilibration on CASA and flow cytometry parameters of thawed ram spermatozoa. Sperm was collected from adult Wallachian rams. The freezing extender was glycerol-free ANDROMED (Minitub GmbH, Tiefenbach, Germany) supplied by 6% exogenous glycerol at different stages of the cryopreservation process.

Possible effects of the MC4R Asp298Asn polymorphism on pig production traits under ad libitum versus restricted feeding.

The missense mutation Asp298Asn in the melanocortin 4 receptor (MC4R) is associated with daily gain or fatness in pigs (Sus scrofa domesticus). However, to the best of our knowledge, no study has directly compared the effects of the polymorphism between different feeding levels, even though diet plays a vital role in the swine industry. To explore possible differences, data from 439 mostly commercial hybrids fattened ad libitum and 119 commercial hybrids fattened with restricted feed ration were collected.

Genetic diversity of two native sheep breeds by genome-wide analysis of single nucleotide polymorphisms.

Wallachian and Sumava sheep are autochthonous breeds that have undergone a significant bottleneck effect and subsequent restoration efforts. The first objective of this study was to evaluate the degree of genetic variability of both breeds and, therefore, the current management of the breeding. The second was to determine whether these two breeds still retain their genetic uniqueness in relation to each other and other breeds, despite regenerative interventions.

Genealogical analysis of European bison population revealed a growing up population despite very low genetic diversity.

In 1919, the European bison population became extinct in the wild. The rescue of the lowland subspecies and the whole species was achieved mainly thanks to individuals from the Białowieża Forest (Polish-Belarusian border). There are currently two breeding lines-the lowland (purebred B.

Sheep Post-Domestication Expansion in the Context of Mitochondrial and Y Chromosome Haplogroups and Haplotypes.

Mitochondrial DNA and nonrecombinant parts of Y-chromosome DNA are a great tool for looking at a species' past. They are inherited for generations almost unaffected because they do not participate in recombination; thus, the time of occurrence of each mutation can be estimated based on the average mutation rate. Thanks to this, male and female haplogroups guide confirming events in the distant past (potential centers of domestication, settlement of areas, trade connections) as well as in modern breeding (crossbreeding, confirmation of paternity).

The Effect of Housing System and Gender on Relative Brain Weight, Body Temperature, Hematological Traits, and Bone Quality in Muscovy Ducks.

The study was conducted during the summer season (June-August 2020). Two hundred sixty-four 5-week-old sexed Muscovy ducklings were randomly divided into four equal experimental groups by housing system and by gender. Each group had three replicates (22 birds/replicate) in a randomized design experiment.

Analysis of Genetic Diversity in the Czech Spotted Dog.

Loss off genetic diversity negatively affects most of the modern dog breeds. However, no breed created strictly for laboratory purposes has been analyzed so far. In this paper, we sought to explore by pedigree analysis exactly such a breed-the Czech Spotted Dog (CSD).