Microarray-based evaluation of selected recombinant timothy grass allergens expressed in E. Coli and N. Benthamiana.

Background: Timothy grass (Phleum pratense) is a significant source of allergens, and recombinant allergens are increasingly used for diagnostic purposes. However, the performance of different recombinant allergen production systems in diagnostic assays needs further investigation to optimize their use in clinical settings.

Objective: The main objective of this study was to analyze and compare the diagnostic performance of recombinant timothy grass allergens produced in E.