Publications by authors named "Karla Y Leyva-Madrigal"
Article Synopsis
- Zucc. (agave tobalá) is a crucial wild species for mezcal production and is primarily found in Oaxaca and Puebla, Mexico; however, a disease outbreak causing dry stem rot has affected 45% of a 10-acre commercial field in Villa Sola de Vega.
- Researchers isolated the pathogen from symptomatic plants by disinfecting tissue samples, culturing them on potato dextrose agar, and identifying several monoconidial isolates with specific morphological characteristics.
- Three of these isolates underwent further tests for phylogenetic analysis and pathogenicity, and were deposited in a culture collection for future study.
Article Synopsis
- * In early 2023, a disease affecting nance trees was reported in Oaxaca, Mexico, with a 50-60% incidence rate of necrotic spots on leaves and fruits.
- * Affected samples were collected and analyzed, leading to the isolation and identification of a pathogen associated with the disease through morphological and molecular analysis, contributing to better understanding of nance fruit health.
Article Synopsis
- Peaches (Prunus persica) are globally popular stone fruits, with 70% of fruits in a Puebla, Mexico orchard showing scab symptoms from 2019 to 2022, characterized by small black lesions.
- The fungus causing these symptoms was isolated using standard lab methods, revealing Cladosporium-like colonies with distinct morphological features consistent with Cladosporium tenuissimum.
- DNA analysis was performed to confirm the identity of the fungus, focusing on specific gene regions through PCR amplification and sequencing, leading to a representative isolate being deposited for further research.
Article Synopsis
- Pitahaya, or dragon fruit, is a cactus native to Mexico, and symptoms of anthracnose were found on its fruit in a commercial orchard in Culiacán, Mexico, in October 2021.
- To isolate the fungus causing these symptoms, researchers disinfested tissue samples and cultured them on potato dextrose agar (PDA), yielding five monoconidial isolates, with one selected for further analysis.
- The selected isolate (CCLF186) underwent morphological identification, phylogenetic analysis, and pathogenicity tests, revealing characteristics consistent with a known species complex and confirming its identity through DNA sequencing techniques.
Article Synopsis
- In November 2018, brown rot symptoms were detected in chayote fruits at the National Germplasm Bank of Chapingo Autonomous University in Mexico, with a 20% disease incidence observed from 120 harvested fruits.
- Fungal isolation involved disinfecting symptomatic fruit pieces and culturing them on potato dextrose agar, resulting in the consistent growth of a specific fungal isolate that showed typical morphological characteristics.
- For molecular identification, genomic DNA was extracted, and the internal transcribed spacer (ITS) region and specific genes were amplified, sequenced, and compared to databases, assisting in phylogenetic analysis to classify the fungus.
Article Synopsis
- Spearmint, an aromatic herb, faced a significant leaf blight disease outbreak in a commercial nursery in Cuautla, Mexico, impacting 1,500 plants with an incidence rate of 89%.
- Fungal isolation from the affected leaves led to the identification of Colletotrichum-like colonies, with one isolate undergoing further testing for pathogenicity and morphological characterization.
- Detailed morphological analysis revealed various features of the isolate, fitting it into the Colletotrichum boninense species complex, and molecular identification was performed through sequencing specific gene regions.
(Fabaceae family), known as guar or clusterbean, is a drought-tolerant annual legume cultivated on a commercial scale focused on industrial gum production. In September 2021, symptoms of leaf spot were observed on guar plants in several commercial fields located at Guasave, Sinaloa, Mexico. Symptoms included round to oval, light brown lesions with dark margins.
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- - Pitahaya, or dragon fruit, is a cactus native to the Americas. In September 2021, a soft rot disease affecting about 15% of the fruit was discovered in a Sinaloa, Mexico orchard.
- - The affected fruits exhibited water-soaked lesions and the presence of fungal mycelia and spores, leading to the isolation of 10 fungal strains, three of which were further characterized and stored for future study.
- - Molecular analysis using DNA sequencing identified these fungal isolates, showing a high similarity to a strain from Taiwan, and a phylogenetic study placed them within a specific fungal clade.
Article Synopsis
- - Observations of collar rot in guar fields in Sinaloa, Mexico revealed symptoms like wilting and reduced growth, with disease incidence between 15% to 40% and a notable presence of white mycelium and brown sclerotia at the plants’ bases.
- - Fungal isolation from diseased plants involved surface sterilization and culturing on PDA medium, resulting in white, cottony colonies with dark brown sclerotia, which were characterized microscopically and purified using the hyphal-tip method.
- - Molecular analysis including DNA extraction and sequencing of the ITS region confirmed the identity of the fungus with 99.21% to 100% similarity to known sequences, and the isolate was deposited in a culture
Article Synopsis
- - Guar is an annual legume from the Fabaceae family, primarily cultivated for industrial use and animal feed, and recently showed anthracnose symptoms in fields in Sinaloa, Mexico, with a disease incidence of up to 15%.
- - The affected plants displayed irregular, necrotic lesions on leaves with a dark brown halo and sunken lesions on pods, leading to the isolation of pathogen colonies which were characterized morphologically and genetically.
- - A specific isolate was deposited for further study, and DNA sequencing of several genes confirmed its identity, matching other known sequences in GenBank, leading to the development of a phylogenetic tree for further analysis.
Fusarium head blight (FHB) is one of the most important diseases affecting wheat production worldwide. In Mexico, and are the dominant species causing FHB of wheat (Cerón-Bustamante et al. 2018).
View Article and Find Full Text PDFSorghum () leaf sheath blight was observed for the first time in Sinaloa, Mexico in the summer of 2020. Fungal isolates were obtained from symptomatic tissue in potato dextrose agar. spp.
View Article and Find Full Text PDFCrop yield and quality are affected by the presence of weeds such as Palmer amaranth. Chemical control is the most commonly used method to eradicate weeds, due to its quickness and efficacy. However, the inappropriate use of chemical herbicides can lead to resistant weed biotypes, as well as problems related to environmental pollution and human health hazards.
View Article and Find Full Text PDFImproved population studies in the fungus Fusarium verticillioides require the development of reliable microsatellite markers. Here we report a set of ten microsatellite loci that can be used for genetic diversity analyses in F. verticillioides, and are equally applicable to other fungi, especially those belonging to the Gibberella fujikuroi clade.
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