Comparative Study of Pain-Related Responses of Male Piglets up to Seven Days of Age to the Application of Different Local Anaesthetics and Subsequent Castration.

To evaluate pain responses to intratesticular and subscrotal injection of three local anaesthetics and their efficacy during castration a randomized controlled study was conducted. In groups of 20 piglets, procaine (2%), lidocaine (2%), or mepivacaine (2%) were administered subscrotal and intratesticularly in two different dosages: 0.5 mL of the original substances or the maximum recommended dosage according to body weight diluted with isotonic saline to a volume of 0.

Investigation on the colonisation of Campylobacter strains in the pig intestine depending on available metabolites.

Campylobacter (C.) spp. represent one of the most important causes for food-borne bacterial pathogen in humans worldwide.

Impact of spp. on the Integrity of the Porcine Gut.

() is the most common food-borne zoonosis in humans, which mainly manifests with watery to bloody diarrhoea. While is responsible for most cases of infection, is less frequently encountered. The object of the study was to prove the clinical impact of mono- and co-colonisation of and on weaned piglets in an infection model and to investigate the impact on transepithelial transport processes in the jejunum and caecum.

Sero- and apx-typing of German Actinobacillus pleuropneumoniae field isolates from 2010 to 2019 reveals a predominance of serovar 2 with regular apx-profile.

Serotyping is the most common method to characterize field isolates of Actinobacillus (A.) pleuropneumoniae, the etiological agent of porcine pleuropneumonia. Based on serology, many farms seem to be infected and antibodies against a wide variety of serovars are detectable, but, so far it is unknown to what degree respective serovars contribute to outbreaks of clinical manifest disease.

Electrocution as an alternative euthanasia method to blunt force trauma to the head followed by exsanguination for non-viable piglets.

Background: On farms, the currently approved and most widely practised method of euthanising non-viable piglets is blunt force trauma to the head followed by exsanguination. However, the use of this method is criticised due to public perceptions and aversion to the methodology by caretakers. Therefore, electrocution after electrical stunning was examined as an alternative approach in 80 hybrid piglets.

Serotyping and pathotyping of Glaesserella parasuis isolated 2012-2019 in Germany comparing different PCR-based methods.

Glaesserella parasuis is an important pathogen in swine production. It acts as a primary pathogen in systemic Glässer´s disease and as a secondary pathogen in Porcine Respiratory Disease Complex. In this study, a collection of 308 isolates from carrier animals and individuals with respiratory or Glässer´s disease isolated 2012-2019 in Germany was analysed.

Validation of a New Resource-Efficient Feeding System for Fattening Pigs Using Increased Crude Fiber Concentrations in Diets: Feed Intake and Ammonia Emissions.

The feeding of fattening pigs and its associated ammonia emissions are current core problems in social debate that affects climate change and sustainability. Feeding methods offer great potential to increase animal welfare and sustainability, and negative impacts on the environment can be reduced. Fattening pigs differ in their performance potential and in their nutrient requirements.

Candidate genes and gene markers for the resistance to porcine pleuropneumonia.

Actinobacillus (A.) pleuropneumoniae is one of the most important respiratory pathogens in global pig production. Antimicrobial treatment and vaccination provide only limited protection, but genetic disease resistance is a very promising alternative for sustainable prophylaxis.

Degraded neutrophil extracellular traps promote the growth of Actinobacillus pleuropneumoniae.

Actinobacillus pleuropneumoniae (A.pp) causes severe pneumonia associated with enormous economic loss in pigs. Peracute diseased pigs die in <24 h with pneumonia.

Treatment With Probiotic Bacteria Does Not Diminish the Impact of a Challenge in Suckling Piglets.

Colonization of newborn piglets with beneficial and ubiquitous microorganisms in combination with colostral passive immunity is the prerequisite for development of immunity and gut maturation. In this study living strains of type A (A) and non-pathogenic (.) strains harvested from healthy piglets were administered to piglets prior to first colostrum intake in order to prevent disease caused by pathogenic variants of the same bacterial species by competitive exclusion.

Multi-organ spreading of Actinobacillus pleuropneumoniae serovar 7 in weaned pigs during the first week after experimental infection.

Actinobacillus (A.) pleuropneumoniae is normally considered strictly adapted to the respiratory tract of swine. Despite this, scattered case reports of arthritis, osteomyelitis, hepatitis, meningitis or nephritis exist, in which A.

Preliminary investigations of spider silk in wounds in vivo - Implications for an innovative wound dressing.

The ideal wound dressing in particular for burn wounds has not been found yet. The aim of this study was to investigate native spider silk as a novel wound dressing. Release of inflammatory cytokines of macrophages and neutrophile granulocytes was determined via ELISA after exposure to spider silk.

Efficacy of a one-shot marbofloxacin treatment on acute pleuropneumonia after experimental aerosol inoculation of nursery pigs.

Background: Porcine pleuropneumonia, caused by is a bacterial respiratory disease of swine. Acute outbreaks of the disease are often accompanied by high mortality and economic losses. As severe cases of the disease frequently require parenteral antibiotic treatment of the animals, the efficacy of a single, high dose of marbofloxacin was compared to a three-time application of a dose of enrofloxacin under experimental conditions.

Treatment of infected lungs by ex vivo perfusion with high dose antibiotics and autotransplantation: A pilot study in pigs.

The emergence of multi-drug resistant bacteria threatens to end the era of antibiotics. Drug resistant bacteria have evolved mechanisms to overcome antibiotics at therapeutic doses and further dose increases are not possible due to systemic toxicity. Here we present a pilot study of ex vivo lung perfusion (EVLP) with high dose antibiotic therapy followed by autotransplantation as a new therapy of last resort for otherwise incurable multidrug resistant lung infections.

Comparison of clinical and immunological findings in gnotobiotic piglets infected with O104:H4 outbreak strain and EHEC O157:H7.

Background: Shiga toxin (Stx) producing () (STEC) is the most frequent cause of diarrhoea-positive haemolytic uraemic syndrome (D + HUS) in humans. In 2011, a huge outbreak with an STEC O104:H4 strain in Germany highlighted the limited possibilities for causative treatment of this syndrome. The responsible STEC strain was found to combine Stx production with adherence mechanisms normally found in enteroaggregative (EAEC).

Porcine pulmonary auto-transplantation for ex vivo therapy as a model for new treatment strategies.

Objectives: Lung auto-transplantation is the surgical key step in experiments involving ex vivo therapy of severe or end-stage lung diseases. Ex vivo therapy has become a clinical reality because of systems such as the Organ Care System (OCS) Lung, which is the only commercially available portable lung perfusion system. However, survival experiments involving porcine lung auto-transplantation pose special surgical and anaesthesiological challenges.

Evaluation of the anaesthetic depth during piglet castration under an automated isoflurane-anaesthesia at farm level.

Piglet castration under isoflurane-anaesthesia could represent an alternative to the practice of castration without anaesthesia. The objective of this study was to evaluate practicality and effectiveness of an automated isoflurane-anaesthesia for castration. A field study on three different farms in Germany (farm A, B: 200 sows; C: 540 sows) was performed.

Actinobacillus pleuropneumoniae challenge in swine: diagnostic of lung alterations by infrared thermography.

Background: Actinobacillus pleuropneumoniae (A.pp.) is the causative agent of porcine pleuropneumonia leading to high economic losses in the pig industry.

Pathway deregulation and expression QTLs in response to Actinobacillus pleuropneumoniae infection in swine.

Actinobacillus (A.) pleuropneumoniae is among the most important pathogens in pig. The agent causes severe economic losses due to decreased performance, the occurrence of acute or chronic pleuropneumonia, and an increase in death incidence.

Labour time required for piglet castration with isoflurane-anaesthesia using shared and stationary inhaler devices.

Isoflurane-anaesthesia combined with an analgesic represents a welfare-friendly method of pain mitigation for castration of piglets. However, it requires an inhaler device, which is uneconomic for small farms. Sharing a device among farms may be an economical option if the shared use does not increase labour time and the resulting costs.

Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine.

Actinobacillus pleuropneumoniae is among the most important pathogens worldwide in pig production. The agent can cause severe economic losses due to decreased performance, acute or chronic pleuropneumonia and an increased incidence of death. Therapeutics cannot be used in a sustainable manner, and vaccination is not always available, but discovering more about host defence and disease mechanisms might lead to new methods of prophylaxis.

In vivo degradation of magnesium alloy LA63 scaffolds for temporary stabilization of biological myocardial grafts in a swine model.

Synthetic or biological patch materials used for surgical myocardial reconstruction are often fragile. Therefore, a transient support by degradable magnesium scaffolds can reduce the risk of dilation or rupture of the patch until physiological remodeling has led to a sufficient mechanical durability. However, there is evidence that magnesium implants can influence the growth and physiological behavior of the host's cells and tissue.

Squamous cell carcinoma in an aged German Landrace sow (Sus scrofa domestica).

Skin tumors are rarely observed in pigs, and, in particular, only a few cases of squamous cell carcinoma have been reported. The present case report describes the occurrence of a squamous cell carcinoma located in the skin at the center of the udder of a 14-year-old German Landrace sow. The tumor was spherical and 17.

Campylobacter spp. - prevalence on pig livers and antimicrobial susceptibility.

The objective of the study was to determine the prevalence of Campylobacter spp. on surfaces of slaughtered pig livers. Multilocus sequence typing (MLST) was performed to determine the sequence types (STs) of selected Campylobacter coli isolates.