Strain development in 3-unit implant-supported CAD/CAM restorations.

Purpose: Passive fit is difficult to achieve in implant-supported restorations with existing superstructure fabrication techniques. The aim of the study presented was to investigate whether computer-generated fixed partial dentures (FPDs) based on optical impressions lead to less strain development than conventionally fabricated FPDs.

Materials And Methods: A measurement model with 2 implants was set up and strain gauges were attached to the model material mesially and distally adjacent to the implants.

Electron probe X-ray microanalysis of intact pathway for human aqueous humor outflow.

Intraocular pressure (IOP) is regulated by the resistance to outflow of the eye's aqueous humor. Elevated resistance raises IOP and can cause glaucoma. Despite the importance of outflow resistance, its site and regulation are unclear.

Neural regeneration and cell replacement: a view from the eye.

Neuronal degenerations in the retina are leading causes of blindness. Like most other areas of the CNS, the neurons of the mammalian retina are not replaced following degeneration. However, in nonmammalian vertebrates, endogenous repair processes restore neurons very efficiently, even after complete loss of the retina.

Endogenous Gas6 and Ca2+ -channel activation modulate phagocytosis by retinal pigment epithelium.

Mutation or loss of MerTK as well as deficiency of alphavbeta5-integrins, gives rise to retinal-degeneration due to inefficient phagocytosis of photoreceptor outer-segment fragments by the retinal pigment epithelium (RPE). This study shows that Gas6 expressed endogenously by human RPE promotes phagocytosis. The RPE expresses Gas6 more highly in vivo and in serum-reduced conditions in vitro than in high-serum conditions, suggesting a negative-feedback control.

Subtype specific estrogen receptor action protects against changes in MMP-2 activation in mouse retinal pigmented epithelial cells.

Eyes with age-related macular degeneration (AMD) demonstrate accumulation of specific deposits and extracellular matrix (ECM) molecules under the retinal pigment epithelium (RPE). AMD is about two times more prevalent in aging postmenopausal women. Therefore we studied whether 17beta-estradiol (E(2)) modulates the expression and activity of the trimolecular complex (MMP-2, TIMP-2 and MMP-14), molecules which are of major importance for ECM turnover in RPE.

Activation of the estrogen receptor contributes to the progression of pulmonary lymphangioleiomyomatosis via matrix metalloproteinase-induced cell invasiveness.

Context: The role of estrogens in the pathogenesis of lymphangioleiomyomatosis (LAM), an aggressive and destructive, eventually fatal lung disease of women, is poorly understood.

Objective: The study was conducted to test the hypothesis that the lung disease in LAM is estrogen mediated and to determine whether estrogens contribute to the invasiveness of LAM.

Design: In vitro cell culture of spindle-shaped LAM cells (LAMD-SM) were isolated and propagated from affected lungs.

Comparative study on interactive computer-aided-learning and computer-aided-testing in patient-based dental training in maxillofacial surgery.

Aim: The objective of this comparative study was to validate, through empirical data, the use of interactive, problem-oriented computer-aided-learning (CAL) and computer-aided-testing (CAT) in dental studies by directly comparing these methods with conventional teaching.

Method: A total of 95 students from the third and fourth clinical semesters participated in an interdisciplinary seminar on oral maxillofacial implantology; 47 of these were taught using conventional teaching methods, while the remaining 48 students were taught using mobiTED, a CAL/CAT-based interactive communication system that can be used patient- and problem-oriented. An examination was given at the end of the course to evaluate student learning.

The effect of load cycling on metal ceramic screw-retained implant restorations with unrestored and restored screw access holes.

Statement Of Problem: It has been shown that under cyclic loading, more chipping fractures of the veneer ceramic occur on the occlusal surface of screw-retained implant-supported fixed partial dentures (FPDs) with unrestored screw access holes (SAHs) than in cemented restorations. This lack of stability may result in esthetic and functional problems.

Purpose: The purpose of this study was to investigate whether, under dynamic loading, fewer chipping fractures occur on the occlusal surface of screw-retained ceramic veneered implant FPDs with restored SAHs than in those with unrestored SAHs.

Parameters of resonance frequency measurement values: a retrospective study of 385 ITI dental implants.

Objectives: There is no proven clinical tool to evaluate the amount of osseointegration and stability around dental implants. Therefore, the aim of this retrospective clinical study was to evaluate resonance frequency analysis values of 385 ITI solid screw implants.

Material And Methods: Both at implant placement and after healing, implant stability quotients (ISQs) were determined.

Influence of fixation mode and superstructure span upon strain development of implant fixed partial dentures.

Purpose: Implant-borne fixed partial dentures (FPDs) should fit passively in order to avoid complications ranging from screw loosening to loss of osseointegration. The aim of this study was to measure the strain development of three-unit and five-unit screw- and cement-retained implant-supported FPDs. Additionally, the influence of the parameters retention mechanism and FPD span were evaluated.

Structural changes in ceramic veneered three-unit implant-supported restorations as a consequence of static and dynamic loading.

Objectives: Static implant loading caused by non-passive restorations may cause technical complications. As metal-ceramic restorations are most common in implant prosthodontics, the objective of the study was to investigate the effect of static and dynamic loading upon the stability of the ceramic veneer of implant-supported fixed partial dentures (FPDs).

Methods: A total of 10 groups of three-unit implant FPDs with five samples each were investigated in the conditions after fabrication, static loading and dynamic loading (chewing simulator, 20,000 cycles, 100N).

Localized and delocalized modes in coupled optical micropillar cavities.

Optical micropillar Bragg cavities of different diameters and coupled by a small bridge have been realized experimentally by means of a focused ion beam system. The resonator modes in these coupled microcavities are either localized in one pillar or delocalized over the whole photonic structure, a fact that could be exploited to control the coupling between two spatially separated quantum dots, i.e.

Different bone loading patterns due to fixation of three-unit and five-unit implant prostheses.

Background: It has been considered that implant prostheses ought to display passive fit. The objective of this finite element analysis (FEA) was to simulate the bone loading resulting from the fixation of implant-supported three and five-unit fixed partial dentures (FPDs).

Methods: Based on a patient case, six different FPD-groups were fabricated using either two or three implants for support.

Gender-specific effects of endogenous testosterone: female alpha-estrogen receptor-deficient C57Bl/6J mice develop glomerulosclerosis.

Young female mice on a C57Bl/6J (B6) background are considered glomerulosclerosis (GS)-resistant but aging B6 mice develop mild GS. Estrogen deficiency accelerates while estrogen replacement retards GS in young sclerosis-prone oligosyndactyly mutant mice on an ROP background. To explore the effects of sex hormones on glomerular structure and function in the context of gender and genetic background, we studied mice in which the estrogen-receptor (ER) genes alpha- or -beta were deleted (alpha- or betaER knockout (KO)) and crossed into the B6 background.

The ternary Rab27a-Myrip-Myosin VIIa complex regulates melanosome motility in the retinal pigment epithelium.

The retinal pigment epithelium (RPE) contains melanosomes similar to those found in the skin melanocytes, which undergo dramatic light-dependent movements in fish and amphibians. In mammals, those movements are more subtle and appear to be regulated by the Rab27a GTPase and the unconventional myosin, Myosin VIIa (MyoVIIa). Here we address the hypothesis that a recently identified Rab27a- and MyoVIIa-interacting protein, Myrip, promotes the formation of a functional tripartite complex.

In vitro effect of load cycling on metal-ceramic cement- and screw-retained implant restorations.

Statement Of Problem: In implant prosthodontics, restorations can either be cement- or screw-retained. Aside from passivity of fit, esthetics, and retrievability, the occlusal opening of the screw-access hole (SAH) is a major difference between the 2 types of restorations. In veneered fixed partial dentures (FPDs), the SAH forms a discontinuity of the ceramic layer and may be an origin for chipping fractures.

Ion channels in the RPE.

In close interaction with photoreceptors, the retinal pigment epithelium (RPE) plays an essential role for visual function. The analysis of RPE functions, specifically ion channel functions, provides a basis to understand many degenerative diseases of the retina. The invention of the patch-clamp technique significantly improved the knowledge of ion channel structure and function, which enabled a new understanding of cell physiology and patho-physiology of many diseases.

Comparison between computer-aided testing and traditional multiple choice: an equivalence study.

Aims: Computer-aided learning (CAL) is a viable alternative to traditional teaching methods. While CAL is used for teaching only, computer-aided testing (CAT) can be applied as a computer-based examination method. The aim of the present study was to compare the performance of undergraduate students in computer-interactive tests and traditional multiple choice tests and to evaluate the influence of question length and language skills on test results.

Cell culture conditions affect RPE phagocytic function.

Background: Changes in the phenotype of retinal pigment epithelium (RPE) cells in vitro are associated with medium conditions and changes in function. Main goals in RPE tissue engineering are cell propagation in serum-free defined culture conditions, resulting in cells exhibiting differentiated morphology and functioning in vitro.

Methods: To compare the effects of various media and supplements on cell function, an optimized high-throughput phagocytosis assay was developed.

Smoking induces glomerulosclerosis in aging estrogen-deficient mice through cross-talk between TGF-beta1 and IGF-I signaling pathways.

Smoking is a known risk factor for the progression of chronic kidney diseases. However, its independent contribution to the development of ESRD and the underlying molecular mechanism have not been well elucidated. Although the risk for ESRD is higher in postmenopausal women according to the US Renal Data System, the number of women who smoke is on the rise worldwide.

Cell-specific differential modulation of human trabecular meshwork cells by selective adenosine receptor agonists.

Activation of A1 and A2A subtype adenosine receptors (AR) likely exert opposing effects on outflow of aqueous humor, and thereby, on intraocular pressure. Selective agonists of adenosine receptor (AR) subtypes have previously been applied to trabecular meshwork (TM) and Schlemm's canal (SC) cells to identify the site(s) of differential purinergic modulation. However, the apparent changes in volume monitored by previously measuring projected cell area might have partially reflected cell contraction and relaxation.

Fixation of 5-unit implant-supported fixed partial dentures and resulting bone loading: a finite element assessment based on in vivo strain measurements.

Purpose: It is believed that implant-supported fixed partial dentures (FPDs) should display passive fit. The objective of this in vivo-based finite element analysis (FEA) was to quantify the magnitude of bone loading occurring on account of the fixation of cemented or screw-retained 5-unit superstructures.

Materials And Methods: Based on a patient situation with 3 implants, 4 different groups of restorations with 10 samples each were fabricated.

Efficient generation of retinal progenitor cells from human embryonic stem cells.

The retina is subject to degenerative conditions, leading to blindness. Although retinal regeneration is robust in lower vertebrates, regeneration does not occur in the adult mammalian retina. Thus, we have developed efficient methods for deriving retinal neurons from human embryonic stem (hES) cells.

Differential effects of continuous and intermittent 17beta-estradiol replacement and tamoxifen therapy on the prevention of glomerulosclerosis: modulation of the mesangial cell phenotype in vivo.

Female ROP Os/+ mice are partially protected by endogenous estrogens against progressive glomerulosclerosis (GS) during their reproductive period; however, ovariectomy accelerates GS progression. We examined the effects of continuous and intermittent 17beta-estradiol (E(2)) replacement and tamoxifen therapy on the development of GS in ovariectomized (Ovx) ROP Os/+ mice. Continuous E(2) replacement (CE(2)) throughout 9 months prevented microalbuminuria and excess extracellular matrix accumulation in Ovx ROP Os/+, not only compared to placebo-treated Ovx mice but also in comparison to intact female ROP Os/+.

Loading of bone surrounding implants through three-unit fixed partial denture fixation: a finite-element analysis based on in vitro and in vivo strain measurements.

Implant-borne fixed partial dentures (FPDs), whether cementable or screwable superstructures, ought to display a true passive fit. The objective of this in vivo-based finite-element analysis is, therefore, to quantify the degree of stress which occurs in the bone around the implants as a result of the fixation of cemented and screw-retained FPDs. On the basis of a simulated patient situation with two implants, six groups of implant-supported superstructures containing 10 samples each were fabricated.