Enrichment of dissolved metal(loid)s and microbial organic matter during transit of a historic mine drainage system.

Water quality degradation by decommissioned mining sites is an environmental issue recognized globally. In the Ore mountains of Central Europe, a wide array of contaminants is released by abandoned under- and aboveground mining sites threatening the quantity and quality of surface and groundwater resources. Here, we focus on the less-explored internal pollution processes within these mines involving organic carbon and microorganisms in trace metal(loid)s mobilization processes.

mRNA expression and protein distribution of the unspliced tenascin-C isoform in prostatic adenocarcinoma.

The inclusion or omission of the alternatively spliced region in the tenascin-C (Tn-C) mRNA gives rise to the large (Tn-C(L)) or small (Tn-C(S)) variant, respectively. Tn-C(L) is thought to be a typical component of provisional extracellular matrices (ECMs) and is expressed during tumour stroma remodelling in cancer. Tn-C(L) mRNA expression and protein distribution have been studied in 44 prostatic adenocarcinomas using RNA/RNA in situ hybridization supplemented by reverse transcriptase-polymerase chain reaction (RT-PCR), and immunohistochemistry (clone BC-2).

Near-infrared fluorescence imaging of HER-2 protein over-expression in tumour cells.

The aim of this study was to evaluate in vitro and in vivo imaging of HER-2-over-expressing tumours using near-infrared optical imaging. A fluorochrome probe was designed by coupling Cy5.5 to anti-HER-2 antibodies.

Differential in vivo and in vitro expression of ED-B+ fibronectin in adult human hematopoiesis.

Fibronectin in general is involved in adhesion and maturation of the erythroid lineage, in megakaryopoiesis and in the differentiation of human multipotent hematopoietic progenitor cells. However, little information exists about the expression of the oncofetal fibronectin isoform containing the ED-B domain (ED-B+ fn) in adult human hematopoiesis. The study was aimed to analyze the ED-B+ fn expression in normal human bone marrow cells by immunocytochemistry, flow cytometry and reverse-transcriptase polymerase chain reaction.

Immunohistochemical demonstration of the gamma2 chain of laminin-5 in urinary bladder urothelial carcinoma. Impact for diagnosis and prognosis.

The heterotrimeric molecule laminin-5 (Ln-5) represents a main protein of the epithelial adhesions complex. It links the basement membrane (BM) with the hemidesmosomes of the basal urothelial cells. The study was aimed to evaluate invasion associated changes of the epithelial adhesion complex in urothelial carcinoma (UC) monitored by immunohistochemical demonstration of the Ln-5 gamma2 chain.