Type 2 pyoverdine from strain BUP2 as - biosensor for the rapid detection of iron and copper ions in contaminated water.

Employing fluorescent quenching mechanism, type 2 pyoverdine (PVD) purified from strain BUP2 (new strain isolated from the rumen of Malabari goat) was used as a simple, convenient and inexpensive tool for the rapid detection of Fe and Cu ions in contaminated drinking water samples. The fluorescence emitted at λ by PVD (in sterile water), mounted on a glass slide was efficiently quenched by the ions of heavy metals like Fe and Cu. The fluorescence quenching effect of PVD was monitored using UV trans-illuminator, and subsequently quantified and confirmed by spectrofluorimetry.