A multi-laboratory validation study was conducted to assess the AOAC OMA 2016.09 method, involving eight labs worldwide that tested six different raw materials and formulated products, all meeting acceptance criteria.
The study focused on evaluating specificity, precision, linearity/range, system suitability, limit of detection (LOD), and limit of quantitation (LOQ), as accuracy and ruggedness had already been validated previously.
Results indicated no major interferences in chromatograms for aloin A, aloin B, and aloe-emodin, with high precision (≤ 0.05 ppm differences) and strong linearity (R ≥ 0.999 across standards), confirming the method's reliability.
