Addition of nitrous oxide to the carbon dioxide pneumoperitoneum strongly decreases adhesion formation and the dose-dependent adhesiogenic effect of blood in a laparoscopic mouse model.

Objective: To evaluate the effect of addition of nitrous oxide (N2O) to the carbon dioxide (CO2) pneumoperitoneum (PP) and the effect of blood, plasma, or red blood cells (RBCs) on postoperative adhesions in a laparoscopic mouse model.

Design: Prospective randomized controlled trial.

Setting: University laboratory research center.

Intraperitoneal temperature and desiccation during endoscopic surgery. Intraoperative humidification and cooling of the peritoneal cavity can reduce adhesions.

This study was conducted to document quantitatively the intraperitoneal temperature and desiccation during laparoscopic surgery. The temperature, relative humidity, and flow rate were measured in vitro and during laparoscopic surgery, at the entrance and at the exit of the abdomen. This permitted us to calculate desiccation for various flow rates using either dry CO(2) or CO(2) humidified with 100% relative humidity at any preset temperature between 25 and 37°C.

Postoperative inflammation in the abdominal cavity increases adhesion formation in a laparoscopic mouse model.

Objective: To investigate acute inflammation in the peritoneal cavity in adhesion formation.

Design: Prospective randomized, controlled trial.

Setting: University laboratory research center.

Intercoat gel (oxiplex): efficacy, safety, and tissue response in a laparoscopic mouse model.

Study Objective: To study the efficacy and safety of Intercoat gel in a laparoscopic mouse model with pneumoperitoneum-enhanced adhesion formation.

Design: Randomized controlled trial. Evidence obtained from a properly designed, randomized, controlled trial (Canadian Task Force classification I).

Effects of adding small amounts of oxygen to a carbon dioxide-pneumoperitoneum of increasing pressure in rabbit ventilation models.

Objective: To evaluate the metabolic consequences of the addition of oxygen to the CO(2)-pneumoperitoneum.

Design: Prospective randomized study in rabbits. After 30 minutes of ventilation pneumoperitoneum was maintained for 90 minutes with pure CO(2) or CO(2) with 2% or 6% of oxygen.

The rabbit nephrectomy model for training in laparoscopic surgery.

Background: Laparoscopic surgical training is generally done with the teacher-student model using complex exercises. This study was performed to evaluate a new training model that emphasizes the repetition of simple procedures.

Methods: Laparoscopic surgery was performed in rabbits (n=200) using conventional instruments.