Liver and pancreatic-targeted interleukin-22 as a therapeutic for metabolic dysfunction-associated steatohepatitis.

Metabolic dysfunction-associated steatohepatitis (MASH) is the most prevalent cause of liver disease worldwide, with a single approved therapeutic. Previous research has shown that interleukin-22 (IL-22) can suppress β-cell stress, reduce local islet inflammation, restore appropriate insulin production, reverse hyperglycemia, and ameliorate insulin resistance in preclinical models of diabetes. In clinical trials long-acting forms of IL-22 have led to increased proliferation in the skin and intestine, where the IL-22RA1 receptor is highly expressed.

Heat illness presentations to emergency departments in Western Sydney: surveillance for environmental, personal and behavioural risk factors.

Objective: To pilot surveillance to describe environmental, personal and behavioural risk factors for people presenting to hospital emergency departments (EDs) with heat illness.

Methods: We conducted a retrospective case series and telephone interview study of people presenting to EDs across South Western Sydney, Western Sydney and Nepean Blue Mountains Local Health Districts with heat illness over the 2017/18 and 2018/19 summer periods (1 December to 28 February). We used the Public Health Rapid Emergency Disease Syndromic Surveillance (PHREDSS) 'heat problems' syndrome to identify people with heat illness and medical records to find contact details.

The Milieu Manager: A Nursing Staffing Strategy to Reduce Observer Use in the Acute Psychiatric Inpatient Setting.

Background: Rising acuity levels in inpatient settings have led to growing reliance on observers and increased the cost of care.

Objectives: Minimizing use of observers, maintaining quality and safety of care, and improving bed access, without increasing cost.

Design: Nursing staff on two inpatient psychiatric units at an academic medical center pilot-tested the use of a "milieu manager" to address rising patient acuity and growing reliance on observers.

Nanocell targeting using engineered bispecific antibodies.

There are many design formats for bispecific antibodies (BsAbs), and the best design choice is highly dependent on the final application. Our aim was to engineer BsAbs to target a novel nanocell (EnGeneIC Delivery Vehicle or EDV(TM)nanocell) to the epidermal growth factor receptor (EGFR). EDV(TM)nanocells are coated with lipopolysaccharide (LPS), and BsAb designs incorporated single chain Fv (scFv) fragments derived from an anti-LPS antibody (1H10) and an anti-EGFR antibody, ABX-EGF.

Characteristics of patients with histories of multiple seclusion and restraint events during a single psychiatric hospitalization.

Background: This descriptive, retrospective study examined patient and event characteristics associated with multiple seclusion and restraint (SR).

Objective: The goal was to identify patient characteristics of multiple seclusion and restraint users to improve patient care and decrease the need for seclusion or restraint.

Design: Medical charts were reviewed for 63 patients hospitalized at the Henry Phipps Psychiatric Service at the Johns Hopkins Hospital between January 2006 and December 2008 who had been secluded or restrained multiple times during a single hospitalization.

Purification of recombinant protein by cold-coacervation of fusion constructs incorporating resilin-inspired polypeptides.

Polypeptides containing between 4 and 32 repeats of a resilin-inspired sequence AQTPSSYGAP, derived from the mosquito Anopheles gambiae, have been used as tags on recombinant fusion proteins. These repeating polypeptides were inspired by the repeating structures that are found in resilins and sequence-related proteins from various insects. Unexpectedly, an aqueous solution of a recombinant resilin protein displays an upper critical solution temperature (cold-coacervation) when held on ice, leading to a separation into a protein rich phase, typically exceeding 200 mg/mL, and a protein-poor phase.

Crisis prevention management: a program to reduce the use of seclusion and restraint in an inpatient mental health setting.

The use of seclusion and restraint in the treatment of mentally ill patients is a highly controversial and potentially dangerous practice. A group of direct care psychiatric nurses in a large urban teaching hospital created an evidenced-based performance improvement program that resulted in a decrease in the use of seclusion and restraint. No additional funds were required to develop this program.

The unit-based CNS: a hand in everything.

Unit-based clinical nurse specialists influence the entire organizational system within a tertiary care setting at Johns Hopkins Hospital, Baltimore, Md.