Publications by authors named "Karin Schann"
Article Synopsis
- * Researchers are engineering microbes like Escherichia coli to utilize formic acid, a reduced one-carbon compound, as their sole source of carbon and energy through the synthetic Serine Threonine Cycle.
- * The study demonstrates that combining tailored strain selection and adaptive laboratory evolution can lead to successful growth using formic acid, highlighting a method for developing complex carbon-assimilation pathways in organisms.
Article Synopsis
- Formaldehyde plays a crucial role in one-carbon metabolism, and improving biosensor technology for its detection is important for enzyme engineering.
- This study created engineered strains of Escherichia coli that require formaldehyde for growth, allowing them to act as sensitive biosensors for various formaldehyde concentrations.
- The developed biosensors not only detect formaldehyde effectively but also have potential applications in enzyme testing and advancing sustainable one-carbon bioeconomy initiatives.
To advance the sustainability of the biobased economy, our society needs to develop novel bioprocesses based on truly renewable resources. The C1-molecule formate is increasingly proposed as carbon and energy source for microbial fermentations, as it can be efficiently generated electrochemically from CO and renewable energy. Yet, its biotechnological conversion into value-added compounds has been limited to a handful of examples.
View Article and Find Full Text PDFAn efficient in vivo regeneration of the primary cellular resources NADH and ATP is vital for optimizing the production of value-added chemicals and enabling the activity of synthetic pathways. Currently, such regeneration routes are tested and characterized mainly in vitro before being introduced into the cell. However, in vitro measurements could be misleading as they do not reflect enzyme activity under physiological conditions.
View Article and Find Full Text PDFEngineering a biotechnological microorganism for growth on one-carbon intermediates, produced from the abiotic activation of CO, is a key synthetic biology step towards the valorization of this greenhouse gas to commodity chemicals. Here we redesign the central carbon metabolism of the model bacterium Escherichia coli for growth on one-carbon compounds using the reductive glycine pathway. Sequential genomic introduction of the four metabolic modules of the synthetic pathway resulted in a strain capable of growth on formate and CO with a doubling time of ~70 h and growth yield of ~1.
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