Ectopic expression of LONELY GUY7 in epidermis of internodal segments for de novo shoot regeneration without phytohormone treatment in ipecac.

In many plant species, the application of exogenous phytohormones is crucial for initiating de novo shoot regeneration. However, ipecac [Carapichea ipecacuanha (Brot) L. Andersson] has a unique ability to develop adventitious shoots on the epidermis of internodal segments without phytohormone treatment.

Increase in ENHANCER OF SHOOT REGENERATION2 expression by treatment with strigolactone-related inhibitors and kinetin during adventitious shoot formation in ipecac.

Increase of ENHANCER OF SHOOT REGENERATION 2 expression was consistent to treatment with kinetin, TIS108, and KK094 in adventitious shoot formation of ipecac. Unlike many plant species, ipecac (Carapichea ipecacuanha (Brot.) L.

Gene expression profiling before and after internode culture for adventitious shoot formation in ipecac.

Background: In ipecac (Carapichea ipecacuanha (Brot.) L. Andersson), adventitious shoots can be induced simply by placing internodal segments on phytohormone-free culture medium.

Strigolactone signaling inhibition increases adventitious shoot formation on internodal segments of ipecac.

SL inhibited adventitious shoot formation of ipecac, whereas the SL-related inhibitors promoted adventitious shoot formation. SL-related inhibitors might be useful as new plant growth regulators for plant propagation. In most plant species, phytohormones are required to induce adventitious shoots for propagating economically important crops and regenerating transgenic plants.

Endogenous auxin determines the pattern of adventitious shoot formation on internodal segments of ipecac.

Endogenous auxin determines the pattern of adventitious shoot formation. Auxin produced in the dominant shoot is transported to the internodal segment and suppresses growth of other shoots. Adventitious shoot formation is required for the propagation of economically important crops and for the regeneration of transgenic plants.