Prospects for Treatment of Lung Cancer Using Activated Lymphocytes Combined with Other Anti-Cancer Modalities.

This review explores the significance and prospects of using diverse T-cell variants in the context of combined therapy for lung cancer treatment. Recently, there has been an increase in research focused on understanding the critical role of tumor-specific T lymphocytes and the potential benefits of autologous T-cell-based treatments for individuals with lung cancer. One promising approach involves intravenous administration of ex vivo-activated autologous lymphocytes to improve the immune status of patients with cancer.

Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay.

Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to give them flexibility, strength and extensibility. However, due to its negative impact on human health, in particular reproductive functions and fetal development, the content of DBP must be controlled in food and the environment. The present study aims to develop a sensitive, fast and simple fluorescence polarization immunoassay (FPIA) using monoclonal antibodies derived against DBP (MAb-DBP) for its detection in open waters.

Sapropel as a Binding Material for Wood Processing Waste in the Development of Thermal Insulation Biocomposite.

When developing new innovative building materials, their performance characteristics as well as their environmental friendliness are important. It is difficult to produce a fully ecological material for building envelopes, because there is a lack of ecological binding materials on the market, good binding materials are very expensive, and cheaper ones have poorer adhesive properties and performance characteristics. In this work, natural organic sapropel was used as an ecological binder.

Colon tumour cell death causes mTOR dependence by paracrine P2X4 stimulation.

Solid cancers exhibit a dynamic balance between cell death and proliferation ensuring continuous tumour maintenance and growth. Increasing evidence links enhanced cancer cell apoptosis to paracrine activation of cells in the tumour microenvironment initiating tissue repair programs that support tumour growth, yet the direct effects of dying cancer cells on neighbouring tumour epithelia and how this paracrine effect potentially contributes to therapy resistance are unclear. Here we demonstrate that chemotherapy-induced tumour cell death in patient-derived colorectal tumour organoids causes ATP release triggering P2X4 (also known as P2RX4) to mediate an mTOR-dependent pro-survival program in neighbouring cancer cells, which renders surviving tumour epithelia sensitive to mTOR inhibition.

Type 2 Diabetes Mellitus: Pathogenic Features and Experimental Models in Rodents.

Type 2 diabetes mellitus (T2DM) is the most common endocrine disorder (90%) in the world; it has numerous clinical, immunological, and genetic differences from type 1 diabetes mellitus. The pathogenesis of T2DM is complex and not fully clear. To date, animal models remain the main tool by which to study the pathophysiology and therapy of T2DM.

Oral Health, Antimicrobials and Care for Patients With Chronic Oral Diseases - A Review of Knowledge and Treatment Strategies.

Periodontal and chronic oral mucosal diseases are significant life impacting conditions which may co-exist and synergistically act to cause more severe and widespread oral pathology with enhanced challenges in effective management. Clinicians regularly observe these effects and struggle to effectively manage both problems in many patients. There is limited understanding of many basic and applied scientific elements underpinning potentially shared aetiopathological features and management.

Overcoming the Limitations of Stem Cell-Derived Beta Cells.

Great advances in type 1 diabetes (T1D) and type 2 diabetes (T2D) treatment have been made to this day. However, modern diabetes therapy based on insulin injections and cadaveric islets transplantation has many disadvantages. That is why researchers are developing new methods to regenerate the pancreatic hormone-producing cells in vitro.

The splicing-regulatory lncRNA NTRAS sustains vascular integrity.

Vascular integrity is essential for organ homeostasis to prevent edema formation and infiltration of inflammatory cells. Long non-coding RNAs (lncRNAs) are important regulators of gene expression and often expressed in a cell type-specific manner. By screening for endothelial-enriched lncRNAs, we identified the undescribed lncRNA NTRAS to control endothelial cell functions.

The human microbiome in immunobullous disorders and lichen planus.

For several decades, there has been a significant growth in the incidence of autoimmune diseases. Studies indicate that genetic factors may not be the only trigger for disease development and that dysbiosis of the microbiome may be another mechanism involved in the pathogenesis of autoimmune diseases. The role of the microbiome in the development of common skin disorders such as psoriasis, atopic dermatitis, acne and rosacea is increasingly well understood.

Effects of Slug Size, Soaking, and Injection Schemes on the Performance of Controlled Ions Water Flooding in Carbonates.

The results of many previous studies on low salinity/controlled ions water (CIW) flooding suggest that future laboratory and modeling investigations are required to comprehensively understand and interpret the achieved observations. In this work, the aim is co-optimization of the length of the injected slug and soaking time in the CIW flooding process. Furthermore, the possibility of the occurrence of several governing mechanisms is studied.

Qualimetric analysis of proton pump inhibitors in Ukraine.

Objective: Introduction: On the pharmaceutical market of Ukraine, there are six international non-proprietary names of proton pump inhibitors (PPIs) - Omeprazole, Pantoprazole, Lansoprazole, Rabeprazole, Esomeprazole, Dexlansoprazole, which differ in a number of pharmacokinetic and pharmacodynamic parameters, safety profile, range of dosage forms and their cost. The aim: To investigate the competitiveness of proton pump inhibitors registered in Ukraine by comparing the parameters of their quality properties using the method of qualimetric analysis.

Patients And Methods: Materials and methods: Qualimetric analysis is based on the deductive-axiomatic approach, which allows quantifying the qualitative properties of drugs and determining the degree of competitiveness of each of them in the pharmaceutical market of Ukraine.

A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination.

Site-specific recombinases (SSR) are utilized as important genome engineering tools to precisely modify the genome of mice and other model organisms. Reporter mice that mark cells that at any given time had expressed the enzyme are frequently used for lineage tracing and to characterize newly generated mice expressing a recombinase from a chosen promoter. With increasing sophistication of genome alteration strategies, the demand for novel SSR systems that efficiently and specifically recombine their targets is rising and several SSR-systems are now used in combination to address complex biological questions in vivo.

Pachydermoperiostosis Masquerading as Acromegaly.

Context: Acromegaly usually is suspected on clinical grounds. Biochemical confirmation is required to optimize therapy, but there are other differential diagnoses.

Case Description: We describe a 24-year-old Uzbek man who presented with many clinical symptoms and signs of apparent acromegaly.

Discovery of Nigri/nox and Panto/pox site-specific recombinase systems facilitates advanced genome engineering.

Precise genome engineering is instrumental for biomedical research and holds great promise for future therapeutic applications. Site-specific recombinases (SSRs) are valuable tools for genome engineering due to their exceptional ability to mediate precise excision, integration and inversion of genomic DNA in living systems. The ever-increasing complexity of genome manipulations and the desire to understand the DNA-binding specificity of these enzymes are driving efforts to identify novel SSR systems with unique properties.

A MARCH6 and IDOL E3 Ubiquitin Ligase Circuit Uncouples Cholesterol Synthesis from Lipoprotein Uptake in Hepatocytes.

Cholesterol synthesis and lipoprotein uptake are tightly coordinated to ensure that the cellular level of cholesterol is adequately maintained. Hepatic dysregulation of these processes is associated with pathological conditions, most notably cardiovascular disease. Using a genetic approach, we have recently identified the E3 ubiquitin ligase MARCH6 as a regulator of cholesterol biosynthesis, owing to its ability to promote degradation of the rate-limiting enzymes 3-hydroxy-3-methyl-glutaryl coenzyme A reductase (HMGCR) and squalene epoxidase (SQLE).

Engineering of a target site-specific recombinase by a combined evolution- and structure-guided approach.

Site-specific recombinases (SSRs) can perform DNA rearrangements, including deletions, inversions and translocations when their naive target sequences are placed strategically into the genome of an organism. Hence, in order to employ SSRs in heterologous hosts, their target sites have to be introduced into the genome of an organism before the enzyme can be practically employed. Engineered SSRs hold great promise for biotechnology and advanced biomedical applications, as they promise to extend the usefulness of SSRs to allow efficient and specific recombination of pre-existing, natural genomic sequences.

Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system.

Targeted genome engineering has become an important research area for diverse disciplines, with site-specific recombinases (SSRs) being among the most popular genome engineering tools. Their ability to trigger excision, integration, inversion and translocation has made SSRs an invaluable tool to manipulate DNA in vitro and in vivo. However, sophisticated strategies that combine different SSR systems are ever increasing.

[Effect of bacteriophage on the lipid peroxidation process and antioxidant protective enzymes in experimental uveitis].

Experimental uveitis features distinct hyperlipoperoxidation in damaged eye tissues, blood serum and the liver. The activity of antioxidant defense (AOD) enzymes decreases in tissues and blood of experimental animals whereas catalase compensatorily activates in hepatic tissue. Experimental therapy of uveitis with gentamycin and bacteriophage results in reducing hyperlipoperoxidation, increased activity of AOD enzymes but no complete normalization is observed.

[Effect of treatment with perftoran on the course of experimental uveitis, lipid peroxidation and activity of antioxidant defense enzymes].

Experimental uveitis is characterized by a pronounced lipid peroxidation (LPO) in damaged tissues of eye, which is complicated by reduced activity of the antioxidant enzymes, SOD and catalase. The intensification of LPO was also found in blood serum and hepatic tissue. However, hepatic lipid peroxidation was accompanied by compensatory increase of catalase activity.

[Disturbance in immunlogic reactivity in rats with uveitis dependeing on the intensity of acetylation processes].

In females and males with uveitis, a similar picture was to be seen of changes in indices for the titre of antibodies in the peripheral blood. It could be summarized that a more profound immunodeficiency in those specimens demonstrating elevated parameters for acetylation is associated with a stronger impact of products of the inflammatory reaction in the eye to immunocompetent cells. But more in-depth research is needed into this question to resolve it.

[The interferon status and cellular immunity of pregnant women with viral hepatitis B].

The examination of 62 pregnant women with acute viral hepatitis B has established in them inhibition of interferon genesis and the presence of secondary T-cell immunodeficiency. The most pronounced and long-lasting was a fall of gamma-interferon. A simultaneous lowering of alpha-, gamma- and serum interferon was indicative of complicated pregnancy or premorbid condition.

[Effect of veroshpiron on hormone level in patients with Parkinson's disease].

Blood hormones and urinary excretion of corticosteroids were measured in 39 parkinsonian patients receiving chemotherapy with adjuvant 3-week course of verospiron (50-100 mg/day). The latter promoted normalization of endocrine and clinical statuses in the majority of the patients. It is suggested that the response may be obtained via neuropeptide systems of the brain.

[A comparative study of the catecholamine content of the blood and urine in patients with facial paraspasm (Meige's syndrome)].

Catecholamines and DOPA excretion and televant levels in blood were measured in 17 patients with facial paraspasm and in 24 healthy subjects. The findings gave evidence for sympathetic-adrenal system adrenal activation and more intensive catecholamine metabolism in patients, especially marked in those with generalised facial hyperkinesia.