Publications by authors named "Karima Boukhors"
Article Synopsis
- 12 out of 220 STEC strains from cattle, food, and children in central France contained the eae gene linked to pathogenicity.
- The study identified 7 different eae pathotypes and described a new variant, espAbetav, while noting associations between specific serogroups and eae alleles.
- Genetic analysis revealed diversity in LEE insertion sites near tRNA genes and indicated the presence of foreign DNA, highlighting the potential health risks these bovine strains may pose to humans.
The gastrointestinal tract of ruminants is the main reservoir for Shiga toxin-producing Escherichia coli (STEC) strains, potentially pathogenic for humans. We used for the first timerumen fluid in which no exogenous carbon source or other supplement was added to compare acid resistance and growth of STEC in physiological physico-chemical conditions. We showed that acidic conditions resulting from the combination of high volatile fatty acid concentration and moderately acidic pH did not alter the survival of STEC, and that human non-O157:H7 STEC isolates were able to persist in the rumen contents in spite of acid stress, low oxygen availability and nutrient deprivation, in the same manner as bovine STEC isolates do.
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