Publications by authors named "Kari H Ecklund"
Using Budding Yeast as a Model to Understand Dynein-Mediated Cargo Transport.
Methods Mol Biol
January 2023
Article Synopsis
- Cytoplasmic dynein-1 is a motor protein that moves along microtubules, facilitating the transport of various cargoes in different cells, but its study has challenges due to its complex roles and regulatory factors.
- Budding yeast serves as an effective model for studying dynein due to its simplified function of positioning the mitotic spindle, allowing for clearer analysis of dynein's activity.
- The paper outlines detailed protocols for using fluorescence microscopy and computational methods to quantitatively measure dynein activity in live yeast cells, offering a valuable resource for researchers interested in this area.
Article Synopsis
- Dynein motors help move the mitotic spindle to the division plane during cell division, with assistance from a microtubule-associated protein (MAP) called She1 in budding yeast.
- She1 plays a crucial role in keeping the spindle near the bud neck, ensuring proper segregation of chromosomes into mother and daughter cells during anaphase.
- The study shows that She1 regulates dynein activity by binding to specific dynein conformations and astral microtubules in the mother cell, thus controlling spindle movement toward the daughter cell.
Cytoplasmic dynein is an enormous minus end-directed microtubule motor. Rather than existing as bare tracks, microtubules are bound by numerous microtubule-associated proteins (MAPs) that have the capacity to affect various cellular functions, including motor-mediated transport. One such MAP is She1, a dynein effector that polarizes dynein-mediated spindle movements in budding yeast.
View Article and Find Full Text PDFThe Kv2.1 voltage-gated potassium channel forms stable clusters on the surface of different mammalian cells. Even though these cell-surface structures have been observed for almost a decade, little is known about the mechanism by which cells maintain them.
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