Equilibrium and non-equilibrium charge-dependent quantification of endothelial cell hydrogel scaffolds.

Using equilibrium swelling and non-equilibrium membrane potential measurements, this study assesses the charge density in two representative series of polyelectrolyte hydrogels and examines the morphological and proliferative responses of endothelial cells as a function of the prepared charge offset. The neutral monomers 2-hydroxyethylmethacrylate (HEMA) and poly(ethylene glycol) dimethacrylate (n = 1,000) (PEGDMA) were copolymerized with either the acidic monomer 2-sulfoethyl methacrylate (SEMA) or the basic monomer methacryloxy ethyltrimethylammonium chloride (MAETAC) to make membranes with pregelation charge offset concentrations varying from 0 to +/-200 mM. A thermodynamic analysis of swelling and membrane potential measurements quantified the hydrogel charge density state following equilibration at different ion strengths.